聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用(英文)

Usage of polyvinyl alcohol filature fibrage in tissue engineering as anterior cruciate ligament scaffolds

背景:单纯的聚乙烯醇材料对细胞的黏附能力有限,用胶原蛋白对其进行表面修饰后能否改善其对细胞的黏附和增殖作用尚无公认。目的:探讨聚乙烯醇纺丝纤维编织用Ⅰ型胶原胶表面修饰后作为构建组织工程前交叉韧带支架材料的可行性。设计、时间和地点:对比观察实验,于2006-08/2007-10在暨南大学医学院第四附属医院,广州市红十字会医院,广州市创伤外科研究所完成。材料:Ⅰ型胶原蛋白胶,由广州市创伤外科研究所研制的胶原胶制剂。方法:用聚乙烯醇纺丝编织成条束状支架材料,用NIH-3T3细胞株和人前交叉韧带细胞体外培养、扩增后,分别种植到聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织(PVA/COL)支架材料上,立体培养。主要观察指标:通过扫描电子显微镜观察NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇与PVA/COL纺丝编织材料上生长及细胞外基质的分泌情况,对比评价编织构建的聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料细胞相容性的优劣。在电子拉力机测试聚乙烯醇纺丝纤维编织支架材料力学性质,用SPSS11.5软件包分析材料的力学性能。结果:NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料表面和孔隙内黏附增殖并能分泌细胞外基质。NIH-3T3细胞株比人前交叉韧带细胞生长旺盛。NIH-3T3细胞株和人前交叉韧带细胞在经I型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料上细胞黏附数量明显增多,在聚乙烯醇支架材料上细胞生长形态较好。I型胶原胶可促进NIH-3T3细胞株分泌细胞外基质但对人前交叉韧带细胞作用不明显。拉力测试该编织材料负荷-拉伸曲线与人及兔前交叉韧带的相似,柔韧性好,最大负荷、极限应力和弹性模量分别为52.61N,14.96MPa和202.08MPa。结论:Ⅰ型胶原可促进NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇纺丝纤维编织支架材料表面和孔隙内黏附、增殖,可促进NIH-3T3细胞株分泌细胞外基质,聚乙烯醇纺丝纤维编织材料具有一定的力学性能和良好的细胞相容性。

BACKGROUND： Simple polyvinyl alcohol （PVA） has limited ability to cell adhesion. There are not generally accepted studies on improved effects of collagen protein modified polyvinyl alcohol on cell adhesion and proliferation. OBJECTIVE： To investigate the feasibility of PVA/type I college （COL- I ） as anterior cruciate ligament （ACL） scaffolds in tissue engineering. DESIGN, TIME AND SETTING： The controlled observation experiment was performed at the Fourth Affiliated Hospital, Medical College, Ji＇nan University, Guangzhou Red Cross Hospital, Guangzhou Institute of Trauma Surgery from August 2006 to October 2007. MATERIALS： COL-I gel was produced by Guangzhou Institute of Trauma Surgery. METHODS： PVA filature was used to weave fascicular scaffolds. NIH-3T3 cell line and human ACL cells were in vitro incubated, amplified, and then implanted on the PVA/COL scaffolds. MAIN OUTCOME MEASURES： The growth of NIH-3T3 cell line and human ACL cells on the PVA/COL scaffolds and the secretion of extracellular matrix were observed using scanning electron microscope. Cell compatibility of PVA/COL scaffolds was assessed. Mechanics characteristic of PVA/COL scaffolds was measured by using the electric tensile force apparatus. Mechanical property of PVA/COL scaffolds was analyzed using the SPSS 11.5 software package. RESULTS： NIH-3T3 cell line and human ACL cells on the PVA/COL scaffolds adhered, proliferated, and secreted extracellular matrix. NIH-3T3 cell line highly grew compared with human ACL cells on the PVA/COL scaffolds. The adhered number of NIH-3T3 cell line and human ACL cells was significantly increased on the PVA/COL scaffolds. NIH-3T3 cell line and human ACL cells presented well morphology on the PVA/COL scaffolds. COL-I could promote the secretion of extracellular matrix from NIH-3T3 cells, but its effects on human ACL cells were not significant. Tensile force test showed that load-extension curve of the materials was identical to ACL of human and rabbits, and the scaffolds possessed strong flexibility. The maximal load, ultimate stress and elastic modulus were respectively 52.61 N, 14.96 MPa and 202.08 MPa. CONCLUSION： COL- I accelerates the adhesion and proliferation of NIH-3T3 cell line and human ACL cells on the surface and in the pore of the PVA/COL scaffolds, promotes the secretion of extracellular matrix from NIH-3T3, and PVA filature material has mechanical property and good cell compatibility.