辐照花粉辅助授粉克服百合受精前障碍的研究

THE OVERCOMING PRE-FERTILIZATION BARRIERS OF LILY VIA ASSISTING POLLINATION OF IRRADIATED POLLEN

采用60Co辐照LA杂种系百合‘耀眼’（‘Dazzle’）花粉,通过辅助授粉克服百合‘Dazzle’（♀）×川百合（Lilium davidii）的远缘杂交障碍,采用荧光显微技术观察授粉后3~7d父本花粉及花粉管在母本雌蕊中的行为。研究结果表明：采用未处理花粉直接授粉,花粉管在花柱中伸长到一定长度后形成胼胝质,停留在花柱中不能进入子房;采用蒙导花粉及先锋花粉授粉法能促进花粉管从花柱进入子房,并接近或可能进入胚珠,表明辐照花粉辅助授粉能部分地克服百合‘Dazzle’（♀）×川百合（Lilium davidii）远缘杂交的受精前障碍。

The effect of irradiated pollen was studied on overcoming incompatibility and incongruity in LA lily cuhivar＇ Dazzle＇ （♀）× Lilium davidii. After 3 - 7 days of pollination, the pollen-tube growth in pistils was observed with fluorescence microscope. The results showed as follows： for normal pollination, pollen tubes stopped growthing in the styles when they strengthened to some length, forming callose at the top of them. For the pollination of mentor pollen and pioneer pollen, pollen tubes entered the ovary, and approached or might penetrated into the ovules. This indicated that the assisting pollination methods with irradiated pollen were able to overcome the pre-fertilization barriers of ＇ Dazzle＇ （ ♀）× Lilium davidii partly.