C57BL/6J小鼠实验性自身免疫性脑脊髓炎模型的建立及其病理特征

Establishment and Pathological Characterization of a C57BL/6J Mouse Model of Experimental Autoimmune Encephalomyelitis

目的探讨C57BL/6J小鼠建立实验性自身免疫性脑脊髓炎(EAE)模型的可能性及其发病特点。方法使用PLP139-151抗原及其C57BL/6J小鼠自制脑脊髓匀浆(spinal cord homogenate,SCH)免疫C57BL/6J小鼠,使用完全福(氏)免疫佐剂为免疫佐剂,并在尾静脉注射百日咳杆菌,建立EAE模型,与经典的PLP139-151免疫的SJL/J小鼠EAE模型进行对比。结果PLP139-151免疫C57BL/6J小鼠仅有一只小鼠表现为尾部张力明显降低;自制SCH免疫C57BL/6J小鼠可见明显脱髓鞘改变。与PLP139-151免疫SJL/J小鼠组相比发病率较低(P<0.05),神经功能评分比较没有明显差异(P>0.05),但发病时间长于PLP139-151免疫SJL/J小鼠组(P<0.05)。结论SCH免疫C57BL/6J小鼠的EAE动物模型,主要表现为急性单相病程,从临床表现和病理学特点来看符合人类MS的病理特点,值得在以后的研究中进一步研究探讨。

Objective To establish an experimental autoimmune encephalomyelitis （EAE） model in C57BL/6J mice, providing a basis for studying the immunopathological mechanism and experimental therapy of multiple sclerosis. Methods Twenty female C57BL/6J mice were immunized with PLP139-151 or spinal cord homogenate to establish the EAE model, and to compare with the classic SJL/J mouse EAE model induced by immunization with PLP139-151. Results The clinic symptoms of EAE were present in only one mouse among the PLP139-151-immunized C57BL/6J mice. Compared with the model group, spinal cord homogenate-immunized C57BL/6J mice had a lower incidence rate （ P 〈 0.05）, but a longer time of disease-induction （ P 〈 0.05）. Light microscopy revealed abundant inflammatory cell infiltration in cerebral and spinal cord tissues in the EAE mice, with evident demyelination in the white matter. Conclusions Spinal cord homogenate-immunization of C57BL/6J mice depicts an acute single phase course, and the animal model bears a resemblance to the features of multiple sclerosis. This mouse model may serve as a better model for the study on multiple sclerosis.