高脂喂养大鼠肝脏的NF-κBp65表达与胰岛素抵抗的相关性

The Relationship between NF-kappaB p65 Expression in Liver and Insulin Resistance in High-Fat Diet Fed Rats

目的探讨高脂饲料喂养大鼠肝脏NF-κBp65蛋白的表达与胰岛素抵抗的关系。方法采用高脂饲料喂养建立胰岛素抵抗大鼠模型,并用正常血糖-高血浆胰岛素钳夹技术评估。应用Western blotting方法检测大鼠肝脏中NF-κBp65蛋白的表达。结果①高脂饲料组大鼠的葡萄糖输注率明显低于基础饲料组[GIR60~120（0.76±0.28vs4.26±0.70）mg/（kg.min）,P〈0.01]。②高脂饲料组大鼠肝脏NF-κBp65蛋白的表达明显高于基础饲料组（A值118.48±1.45vs68.13±4.84,P〈0.01）。③高脂胰岛素抵抗大鼠肝脏NF-κBp65蛋白表达与GIR60-120（r=-0.993,P=0.000）和ISI（r=-0.773,P=0.009）负相关。结论高脂诱导的胰岛素抵抗大鼠肝脏NF-κB的激活可能是产生肝脏和全身胰岛素抵抗的根源。

Objective To investigate the relationship between NF-kappaB p65 expression in liver and insulin resistance in rats induced by high-fat diet feeding. Methods Insulin resistant rat model was induced by high-fat diet feeding. Twenty male Wistar rats were randomly divided into two groups fed with either basic chow （NC,n = 10） or high-fat diet （HF,n = 10） for 10 weeks, then assessed by euglycemic-hyperinsulinemia clamp technique. The NF-kappaB p65 expression was tested by Western blotting. Results 1. The glucose infusion rate （GIR） in HF group decreased significantly compared with that in NC group [ GIR60-120 （0.76± 0.28 versus 4.26 ± 0.70） mg/（kg· min）, P 〈 0.01 ]. 2, The NF-kappaB p65 expression of liver in HF group was significantly increased in comparison with that in NC group （ A value 118.48 ± 1.45 versus 68.13 ± 4.84, P 〈 0.01 ）. 3. The NF-kappaB p65 expression in liver of insulin resistant rats was negatively correlated with either GIP60-120 （ r = - 0. 993, P = 0.000） or insulin sensitivity index （ISI） （ τ = - 0.773, P = 0.009）. Conclusion The activation of NF-kappaB in liver of insulin-resistant rats induced by high-fat diet feeding probably results in hepatic and systemic insulin resistance.