SPF级新生大鼠高氧肺损伤模型的建立

Establishment of SPF Neonatal Rats Model of Hyperoxic Lung Injury

目的建立一种操作简便、性能稳定的新生大鼠高氧肺损伤动物模型。方法①设计制造能自动控制氧浓度的高氧动物饲养舱。②将孕期满21 d出生的SPF级新生大鼠随机分成4组,即：高氧组Ⅰ（入高氧舱中饲养1～7 d）,高氧组Ⅱ（入高氧舱中饲养14 d）,以及相应的空气对照组Ⅰ、Ⅱ,每组14只。舱内氧浓度≥90%,每天23 h。计算肺系数,HE染色与病理学观察。结果高氧组与相应的对照组比较：高氧组大鼠体重轻,肺系数大,HE染色显示部分肺泡萎缩、肺间质及肺泡腔有水肿、出血,中性粒细胞浸润;肺泡发育明显滞后,辐射状肺泡计数明显少。结论本动物饲养舱性能稳定,操作简便;复制的新生大鼠的肺病理变化符合高氧肺损伤的改变。

Objective To establish a stable neonatal rat model of hyperoxic lung injury with a simple procedure. Method 1 ） To design and construct an automatically controlling oxygen density hyperoxia animal feeding cabin. 2） SPF neonatal rats born at 21 days of gestation were divided into 4 groups： hyperoxia group Ⅰ （fed in the hyperoxic cabin for 1 - 7 d） ,hyperoxia group Ⅱ （fed in the hyperoxic cabin for 14 d）, and corresponding air control group Ⅰ and Ⅱ , 14 rats per each group. The oxygen density in the cabin was kept ≥ 90 %, 23 hours per day. Histopathological changes of the lung tissues were examined by HE staining and the lung factor was calculated. Result To compare the hyperoxia groups with corresponding air control groups, the rats of hyperoxia groups showed deceased body weight, greater lungs factor, partial alveolar atrophy, hemorrage and edema in the pulmonary interstitium and alveolar cavity, neutrophil infiltration, evident retardation of alveolar growth, and distinctly decreased radial alveolar count. Conclusion The animal feeding cabin described in this study is simple to construct and with stable performance. The pulmonary pathological changes in neonatal rats fed in this feeding system are well consistent with those of hyperoxic lung injury.