大鼠慢性胰腺炎离体组织块高分辨氢质子磁共振波谱分析

The metabolite features of chronic pancreatitis in rats

目的对CP大鼠离体胰腺组织块进行高分辨磁共振波谱分析，探索其代谢变化特征。方法SD大鼠30只，数字随机法分为实验组（20只）与对照组（10只）。实验组经尾静脉注射二丁基二氯化物8mg／kg体重，对照组注射等量溶剂。胰腺组织行病理检查及胶原纤维染色。运用高分辨魔角旋转磁共振波谱技术对离体大鼠CP组织样品代谢物进行分析。结果造模60d后，11只大鼠胰腺小叶结构破坏，腺泡消失，炎症细胞广泛浸润，纤维组织大量增生，广泛间质纤维化，呈典型CP表现。波谱分析显示CP组织的磷酸胆碱（PC）和甘油磷酸胆碱（GPC）、牛黄酸（Tau）及乳酸（Lac）峰强度增加；甜菜碱（Bet）、谷氨酸（Glu）、丙氨酸（Ala）、异亮氨酸（Ile）、亮氨酸（Leu）、缬氨酸（Val）峰降低；而乙酸（Ace）、胆碱（Cho）峰强度则无明显差异。结论离体大鼠CP组织具有显著的代谢特征，为人CP波谱分析研究奠定了实验基础。

Objective To investigate the metabolite features of chronic pancreatitis in rats in vitro by high resolution magic angle spinning nuclear magnetic resonance spectroscopy. Methods A total of 30 Spraque-Dawley rats were randomized into experimental group （n = 20 ） and control group （n = 10 ）. All the animals in experimental group were intravenously injected with 8 mg/kg body weight DBTC, and the animals in the control group received same amount of solvent. Pancreatic tissues were examined by histology and Van Gieson staining. Metabolic changes of chronic pancreatitis in vitro in rats were studied by high resolution magic angle spinning nuclear magnetic resonance spectroscopy. Results 60 days after DBTC application, the pancreatic tissue was characterized by an extended interstitial fibrosis with infiltrating mononuclear cells. Compared with the control group, the signal intensities of phosphocholine （ Pc ） and glycerophosphocholine （Gpc）, tanfine （Tau）, lactate （Lae） of chronic pancreatitis group increased. Oppositely, the signal intensities of betine （Bet）, glutamic acid （Glu）, alanine （Ala）, ileucine （Ile）, leucine （Leu） and valine （Val） decreased. The signal intensities of acetic acid （Ace） and choline （Cho） were not changed. Conclusions There were obvious metabolic features of chronic pancreatitis in rats, and it is helpful for the application of magnetic resonance spectroscopy in chronic pancreatitis in human studies.