大豆提取物(CKBN)对免疫低下小鼠免疫功能的影响

Effect of a soybean Extract(CKBN) on Immune Function in Immunosuppressed Mice

目的观察大豆提取物(CKBN)对免疫低下小鼠免疫功能的影响。方法腹腔注射环磷酰胺(cyclophosphamide,CTX)建立免疫功能低下小鼠模型,观察1、25、50、100 mg/kg剂量CKBN对免疫低下小鼠免疫功能的影响。结果25 mg/kg组的CKBN可显著增加免疫低下小鼠的脾指数;25、50、100 mg/kg组均能明显抑制环磷酰胺对小鼠外周血白细胞数量的影响,1 mg/kg组可显著提高单核细胞百分率,50 mg/kg组可显著提高中性粒细胞百分率;100 mg/kg组的IgG2a水平高于环磷酰胺组;1、25、50 mg/kg可显著提高腹腔巨噬细胞的吞噬功能;25 mg/kg组可显著提高NK细胞杀伤活性。结论CKBN能显著增强CTX造成的免疫低下小鼠的免疫功能。

Objective To investigate the effect of a soybean extract（CKBN） on immune function in immunosuppressed mice. Methods Immunosuppressed mice were induceded by intraperitoneal injection of cyclophosphamide （CTX）. The effect of CKBN treatment at a dose of 1 mg/kg, 25 mg/kg, 50 mg/kg, and 100 mg/kg on the immune function in CTX-induced immunosuppressed mice was studied. Results The spleen index was significantly increased in the group of mice treated with 25 mg/kg CKBN, respectively. The inhibitory effect of CTX on the peripheral leucocyte count was revessed by the treatment of 25 mg/ kg, 50 mg/kg, and 100 mg/kg CKBN. The percentage of mononuclear cells was markedly raised by 1 mg/kg CKBN treatment, and the percentage of neutrophils was significantly increased in the 50 mg/kg CKBN treatment group. IgG2a level in the 100 mg/ kg CKBN treatment group was higher than that in the CTX group. There was an induction of phagocytic activity of peritoneal macrophages in the groups with 1 g/kg, 5 mg/kg, and 50 mg/kg CKBN treatment in comparison with that in the CTX group. The activity of NK cells was remarkably stimulated in the group treated with 25 mg/kg CKBN. Conclusion CKBN significantly increases the immune function in CTX-induced immunosuppressed mice.