猪小肠移植术供肠的灌洗和保存

Perfusion and Preservation of Isolated Piglets Small Intestine Graft for Transplantation

目的探讨围手术期移植小肠灌注和保存的方法。方法切取猪供肠后,采用100 cm左右高度、略加压法,经移植肠血管以15 mL/min左右灌洗速度持续灌注4℃3%羟乙基淀粉注射液、4℃生理盐水保存移植肠。移植前对保存的移植小肠进行组织学检查。结果供肠总灌注时间为50.5±10.6 min;冷缺血时间为80.24±24.62min。组织学检查显示移植肠组织学没有明显改变。移植肠存活良好。结论采取上述方法在短时间内可以提供质量良好的供肠。

Objective To investigate the perfusion and preservation of small intestine graft during perioperation period in piglets small intestinal transplantation. Methods The graft was treated with a continuous intra-arterial 3 % hydroxyethyl starch solution perfusion. The perfusion was carried out at a height of 100 cm and flow rate of 15 mL/min. The intestine graft was preserved in 0.9% normal saline at 4℃ . The intestine graft was assessed by histology before transplantation. Results The perfusion and cold ischemia time lasted 50.5 ± 10.6 min and 80.24 ± 24.62 min, respectively. No change was observed in the small intestine graft before transplantation. The transplanted small intestine survived well. Conclusion The above described measures can well maintain the structure and function of the small intestine graft in short time and satisfy the demand of small intestine transplantation.