桂枝芍药知母汤对免疫性关节炎大鼠TNF-α与Bcl-2表达的影响

Effect of GSZ Decoction on TNF-α and Bcl-2 Expression in Immunologic Arthritis Rats

目的:观察桂枝芍药知母汤对免疫性关节炎大鼠血清中TNF-α与关节滑膜组织Bc l-2表达的影响,并初步探讨其治疗机制。方法:以弗氏完全佐剂诱导产生免疫性关节炎模型,用不同剂量桂枝芍药知母汤(GSZ汤20.6、10.3 g/kg)给模型动物灌胃用药,观察用药后动物血清TNF-α水平与致炎对侧膝关节滑膜组织Bc l-2表达。结果:桂枝芍药知母汤用药4w后,可明显使关节指数减小(P<0.05或P<0.01);使模型大鼠血清TNF-α水平降低(P<0.01);免疫组化染色镜检显示,大鼠膝关节滑膜组织Bc l-2阳性细胞数减少、阳性细胞着色强度减弱(P<0.05或P<0.01),本实验条件下,GSZ汤20.6 g/kg剂量时的疗效与MTX相当。结论:桂枝芍药知母汤对免疫性关节炎大鼠有治疗作用,其机制可能与抑制TNF-α分泌,降低Bc l-2表达有关。

Objective ：To observe the therapeutic effect of guizhi-shaoyao-zhimu （GSZ）decoction on immunologic arthritis rats and investigate the mechanism by determining the expression of serum TNF-α and Bcl-2 in synovial tissues of joint. Methods：The rats were prepared for immunologic arthritis models by Freund＇s complete adjuvant（FCA）. Different doses of GSZ decoction （20. 6 g/kg, 10. 3 g/kg） were administrated on rat models. The serum of experimental animals were collected to detect TNF-α, and the counter-lateral synovial tissues of the knee joint were taken for observing the expression of Bcl-2 by immunohistochemical test. Results ： In a 4-week administration of GSZ decoction,lessened arthritis index was observed （P 〈 0. 05 or P 〈 0. 01 ） ,which resulted in a lower serum TNF-α in the model rats （ P 〈 0. 01 ） ;The immunohistochemical test suggested Bcl-2 positive staining cell count became smaller and weakened （P 〈0. 05 or P 〈0.01）. Moreover,the findings suggested that GSZ decoction 20. 6 g/kg could be favorable with respect to MTX in treatment of immunologic arthritis rats. Conclusion： GSZ decoction can treat the joint impairment of immunologic arthritis rats. The therapeutic mechanism may be relevant to reducing the content of TNF-α and inhibiting the expression of Bcl-2.