西洋参中皂苷类成分提取方法对比

Comparison Resarch on Different Extraction Methods of Ginsenosides from Panax quinquefolium

目的:选择更好的西洋参提取方法,提高人参皂苷的提取率。方法:以人参总皂苷、人参皂苷Rb1、Rb2、Rc、Rd、Re、Rg1、Rg2等7种主要单体皂苷收率为指标,用高效液相色谱法进行含量测定,比较热回流提取、超声波提取、罐组式逆流提取、组罐式超声波提取四种方法各自的优劣。结果:组罐式超声波提取是最佳的提取方法,它可以在保证提取效率的前提下,减少溶剂的用量,降低提取温度。结论:组罐式超声波提取法可望广泛地应用在制药工业上。

Objective： To optimize the extraction method and improve the extraction ratio of ginsenosides from Panax quinquefolium. Methods： Four different extraction methods, including heat reflux extraction method, ultrasonic extraction method, multi-stage countercurrent extraction method and uhrasonic-assisted muhi-stage countercurrent extraction method were compared according to the extraction ratio of total saponins and seven ginsenosides analyzed by HPLC including Rb1, Rb2, Rc, Rd, Re, Rg1, Rg2. Results： The ultrasonic-assisted multi-stage countercurrent extraction method was the best method and had the advantages of higher efficiency, lower extraction temperature and less solvent consumption. Conclusion： The ultrasonic-assisted multi-stage countercurrent extraction method is hoped to be widely used in the pharmaceutical industry.