中药白根独活中总香豆素成分的含量测定

Determination of Total Coumarins in Angelica Polymorpha Maxim.

建立紫外分光光度法测定白根独活中总香豆素的含量．方法：白根独活药材粉末经超声波提取，采用紫外分光光度法，检测波长为303．7nm对白根独活药材中的总香豆素进行测定．结果：采用该方法可准确测定白根独活药材中总香豆素的含量，水合氧化前胡素在6．4～19．2μg／mL范围内，吸光度与浓度的线性关系良好（r=0．9991），样品平均回收率为98．53％，精密度RSD为0．17％，稳定性RSD为0．37％（5h），重复性RSD为O．55％．结论：该方法简单、准确，可用于白根独活药材中总呋喃香豆素的含量测定．

An UV spectrophotometry for determining total coumarins in Angelica polymorpha Maxim. is established. The powder of Angelica polymorpha Maxim. is extracted by ultrasonic technology. The total coumarins is detected at 303.7 nm by UV spectrophotometry. There is a good linear relationship within the range of 6.4-19. 2 μg/mL（r=0. 999 1）. The relative standard deviation of accurate test and stability test are 0.17% and 0. 37% respectively. The average recovery is 98.53%（n=6） with RSD of 0.55%. The method is simple and accurate; and it can be used for determining of the total coumarins in Angelica polymorpha Maxim..