以钴离子为探针测定半胱氨酸的研究

Cysteine Determination Using Cobalt (Ⅱ) Ionprobe

详细研究了半胱氨酸与钴（Ⅱ）在ＮＨ４ＣＬＮＨ４ＯＨ介质中的相互作用，建立了快速测定半胱氨酸的分光光度法，方法检出限２８×１０－６ｍｏｌ·Ｌ－１，线性范围０～２０×１０－４ｍｏｌ·Ｌ－１，摩尔吸光系数７９８×１０３Ｌ·ｍｏｌ－１·ｃｍ－１。用于尿液中半胱氨酸的测定，结果满意。

Based on the reaction of cysteine and cobalt (Ⅱ) in NH 4Cl NH 4OH solution, a new spectrophotometric method for the determination of cysteine was developed. The molar absorptivity is 7.98×10 3 L·mol -1 ·cm -1 at 284 nm and the results met the Beer′s law in the range of 0～2.0×10 -4 mol·L -1 . The method was applied successfully in cysteine assay in urine.