红景天苷对不同状态下小鼠能量代谢的影响

Effect of Salidroside on Energy Metabolism of Mice

目的探讨红景天苷(SDS)对不同状态下小鼠能量代谢的影响。方法将32只小鼠随机分为对照组、SDS组、运动组及SDS+运动组4组,其中SDS组及SDS+运动组给予180mg/(kg·d)SDS灌胃,对照组及运动组则给予同体积蒸馏水[20ml/(kg·d)]灌胃,连续给药15天。15天后对照组及SDS组不做任何运动,运动组及SDS+运动组无负重游泳120分钟后处死小鼠,检测骨骼肌组织匀浆中苹果酸脱氢酶(MDH)、琥珀酸脱氢酶(SDH)、丙酮酸激酶(PK)及乳酸(LD)和肝组织匀浆中乳酸脱氢酶(LDH)水平。结果SDS+运动组小鼠骨骼肌内MDH活性明显高于对照组(P<0.05);SDS组、运动组和SDS+运动组骨骼肌内SDH活性分别较对照组升高13%(P>0.05)、16%(P<0.05)和27%(P<0.01),且SDS+运动组骨骼肌内SDH活性也明显高于运动组(P<0.05);运动组和SDS+运动组骨骼肌内PK活性较对照组分别升高14%和39%(P均<0.05),且SDS+运动组骨骼肌内PK活性也明显高于运动组(P<0.05)。SDS组、运动组和SDS+运动组小鼠肝脏LDH活性较对照组分别升高17%(P<0.05)、16%(P<0.05)和28%(P<0.01),且SDS+运动组的肝脏LDH活性也明显高于运动组(P<0.05);运动组骨骼肌内LD含量明显高于对照组(P<0.05)。结论SDS可增加运动小鼠骨骼肌及肝脏中能量代谢相关酶活性,促进有氧代谢和加速骨骼肌内LD清除可能是SDS抗运动性疲劳的机制之一。

Objective To study the effect of salidroside （SDS） on energy metabolism of mice. Methods Totally 32 normal male Kunming mice were randomly divided into control group, SDS group, exercise group, and SDS plus exercise group with 8 mice in each group. The control group and exercise group were given distilled water and the SDS group and salidroside plus exercise group were given salidroside （ 180 mg/kg · bw） for 15 consecutive days. The activity of skeletal muscle malate dehydrogenase （MDH） , succinate dehydrogenase （SDH） , pyruvate phosphokinase （PK） , and hepatic lactate dehydrogenase （LDH） were determined. The content of skeletal muscle lactic acid （LD） was detected. Results The MDH activity was significantly higher in the SDS plus exercise group than in the control group. The SDH activities in SDS group, exercise group, and SDS plus exercise group were increased by 13% （P〉0.05）, 16% （P〈0.05）, and 27% （P〈0.01） when compared with the control group. Meanwhile, the SDH activity inside skeletal muscles was significantly higher in SDS plus exercise group than in exercise group （ P 〈 0.05 ）. The PK activities inside skeletal muscles in exercise group and SDS plus exercise group were increased by 14% and 39% when compared with the control group （ both P 〈 0. 05 ） ; meanwhile PK activity in SDS plus exercise group was also significantly higher than in exercise group （ P 〈 0.05）. The liver LDH activities in SDS group, exercise group, and SDS plus exercise group increased by 17% （P 〈 0.05）, 16% （P 〈 0.05 ）, and 28% （ P 〈 0.01 ）, respectively, when compared with the control group; meanwhile, the liver LDH activity in SDS plus exercise group was significantly higher than in exercise group. The LD level inside the skeletal muscles was significantly higher in exercise group than in control group （ P 〈 0.05 ）. Conclusion The antifatigue effects of SDS in mice may be correlated with improved oxidize metabolic capability.