特异性免疫治疗对特应性皮炎T细胞亚群及NK细胞的影响

Effects of specific immunotherapy on the T subpopulation and natural killer cell in atopic dermatitis

目的：探讨特异性免疫治疗对特应性皮炎T细胞亚群及NK细胞的影响。方法：采用流式细胞仪检测45例特异性皮炎（atopic dermatitis，AD）患者和30例健康献血员外周血CD3^＋、CD4^＋、CD8^＋T细胞、B细胞、NK细胞的构成比，计算CD4／CD8比值；并比较27例AD患者经特异性免疫（specific immunotherapy，SIT）治疗后T细胞亚群、NK细胞的变化。结果：AD患者CD4^＋、NK细胞构成比、CD4^＋/CD8^＋比值均低于正常对照组（P〈0．05），而CD3^＋、CD8^＋、B细胞与正常组相比差异无统计学意义（P〉0．05）。AD患者经SIT治疗6～18个月后，CD4^＋、NK细胞构成比、CD4^＋／CD8^＋比值均高于治疗前患者，差异有统计学意义（P〈0．05）。结论：AD患者存在细胞免疫功能低下，SIT治疗可显著提高AD患者细胞免疫功能，对AD患者不失为较为有效的对因治疗方法。

Objective： To study the influence of specific immunotherapy on the T subpopulation and natural killer cell in atopic dermatitis （AD）. Methods： CD^3＋ , CD^4＋ , CDs T cell, B cell and NK cell of the peripheral blood in 45 patients with atopic dermatitis and 30 sex and age matched controls were assayed by flow cytometry, CD4/CD8 was calculated, T subpopulation and natural killer cell of 27 patients with AD were assessed after specific immunotherapy. Results：The percentage of CD4^＋ , NK cell and CD4^＋/CD8^＋ were significantly lower in the patients with AD than that in the control group （ P 〈 0.05 ）. For CD3^＋, CD8^＋ T cell and B cell, there were no obvious distinguish between AD and the control group （P 〉 0. 05 ）. The percentage of CD4^＋ , NK cell and CD4^＋ / CD8^＋ increased significantly after 6 to 18 months specific immunotherapy. Conclusion：The cellular immune function is reduced in the AD patients. The specific immunotherapy can improve cellular immune function effectively.