过氧化物酶体增殖物激活受体δ＋294T／C基因多态性与冠心病的关系

Relationship of peroxisome proliferation-activated receptor-delta+294T/C gene polymorphism with coronary artery disease

目的探讨过氧化物酶体增殖物激活受体δ(PPARδ)+294T/C基因多态性与冠心病的关系。方法运用聚合酶链式反应及限制酶切片段长度多态性技术分析无血缘关系汉族人群[82例正常对照者(NC),120例冠心病(CHD)患者]的PPARδ+294T/C基因多态性,分析基因型频率、等位基因频率,并对不同基因型患者冠心病的危险性进行评价。结果两组间基因型分布有显著性差异(P<0.05);CHD组TC+CC基因频率及C等位基因频率均明显高于NC组(P<0.05);C等位基因携带(TC+CC)者冠心病危险性高于TT基因型(C等位基因携带者OR:3.16,95%CI:1.39～7.14)。结论PPARδ+294T/C基因多态性与冠心病之间有重要的相关性,C等位基因携带可能是冠心病的一个的危险因素。

Objective To investigate the relationship of peroxisome proliferation-activated receptor-delta （PPARδ） ＋ 294T/C gene polymorphisms with coronary heart disease （CHD）. Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls（NC） and 120 cases diagnosed as CHD. PPARδ ＋ 294T/C gene polymorphisms were determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ ＋ 294T/C genotypes and the ＂C＂ allele frequency were analyzed, to evaluate the risk of the CHD among variant genotypes. Results The genotype distribution was significantly different between the CHD and the NC （P 〈 0.05 ）. The frequencies of TC ＋ CC genotype and ＂C＂ allele were eridently higher in the CHD group than in the NC, the CHD risk of the＂ C＂ allele carriers was significantly higher than that of the TT homozygote （ ＂C＂ allele OR ： 3.16,95 % CI： 1.39 - 7. 14 ）. Conclusion There is evident association between PPARδ ＋ 294T/C gene polymorphisms and CHD. The ＂C＂ allele may be a risk factor of CHD.