MCL1蛋白在胃癌中的表达及临床意义

Expression of MCL1 Protein in Gastric Carcinoma and Its Clinical Significance

目的:MCL1(myeloid cell leukemia-1)与多种恶性肿瘤的发生、发展及转移相关。本文从临床样本和细胞水平检测胃癌组织中MCL1蛋白的表达水平,探讨MCL1蛋白在胃癌发生、发展中的作用。方法:采用S-P免疫组化法检测正常胃黏膜、癌旁胃黏膜和胃癌组织芯片中MCL1蛋白的表达,Western blot检测MCL1蛋白在人胃癌BGC823、SGC7901、MKN28细胞中的表达。结果:免疫组化结果显示,MCL1蛋白表达定位于细胞的胞浆,着色呈黄色至棕黄色;正常胃黏膜、癌旁胃黏膜、胃癌中MCL1蛋白阳性表达率分别为18.18%(4/22)、54.17%(26/48)、83.33%(75/90);MCL1蛋白在胃癌中的表达高于癌旁胃黏膜及正常胃粘膜,癌旁胃黏膜与正常胃粘膜MCL1蛋白表达亦具有明显差异(P<0.05);MCL1蛋白阳性表达率在伴淋巴结转移组为93.48%(43/46),无淋巴结转移组为72.72%(32/44),两者差异有显著性意义(P<0.05);而与性别、年龄和肿瘤大小无关。S-P免疫组化法与Western blot结果显示MCL1蛋白表达与胃癌分化程度呈负相关。结论:MCL1蛋白表达与胃癌发生、分化程度和淋巴结转移相关。

Objective： To investigate the expression of MCL1 protein in gastric carcinoma and to explore the relationship between MCL1 protein expression and the development of gastric carcinoma. Methods： Expression of MCL1 protein was examined immunohistochemically by the streptavidin-peroxidase conjugated method （SP） in normal mucosa, paracancerous tissues and gastric carcinoma. Western blot was used to detect the expression of MCL1 protein in BGC823, SGC7901 and MKN28 gastric cancer cell lines. Results： MCL1 protein expression was located in the cytoplasm. The positive rate of MCL1 protein expression was 18.18% （4/22） in normal gastric mucosa, 54.17% （26/48） in paracancerous tissues, and 83.33% （75/90） in gastric carcinoma tissues. The positive rate of MCL1 protein expression in gastric carcinoma was significantly higher than that in paracancerous tissues （P〈0.05）. The positive rate of MCL1 protein expression in tissues adjacent to carcinoma lesions was significantly higher than that in normal gastric mucosa （P〈0.05）. MCL1 expression rate was 93.48% （43/46） in tissues from carcinoma patients with lymph node metastases and 72.72% （32/44） in those without lymph node metastases, with a significant difference （P〈0.05）. No correlation was found between MCL1 protein expression and gender or age. The expression of MCL1 was negatively correlated with the differentiation of gastric carcinoma. Conclusion： MCL1 protein expression is related to the carcinogenesis, differentiation and lymph node metastasis of gastric carcinoma.