氨氯地平对氧化型低密度脂蛋白诱导的平滑肌细胞亲环素A表达的影响

Effect of Amlodipine on Cyclophilin A Expression in Smooth Muscle Cells Induced by Oxidized Low Density Lipoprotein

目的观察氨氯地平对氧化型低密度脂蛋白诱导的平滑肌细胞亲环素A表达的影响,以进一步探讨氨氯地平对动脉粥样硬化的作用机制。方法用不同浓度氨氯地平(0、0.1、1.0和10.0μmol/L)预孵育平滑肌细胞1h后,与氧化型低密度脂蛋白共同孵育,逆转录聚合酶链反应和Western blotting法检测细胞亲环素AmRNA及蛋白的表达。结果氧化型低密度脂蛋白抑制平滑肌细胞亲环素AmRNA及蛋白的表达,而氨氯地平预处理可上调亲环素A的表达。结论氨氯地平可逆转氧化型低密度脂蛋白抑制平滑肌细胞亲环素A表达的作用。

Aim To investigate the effect of amlodipine on the expression of Cyclophilin A in smooth muscle cells induced by oxidized low density lipoprotein （ox-LDL） , so as to approach the mechanism of amlodipine in atherosclerosis. Methods Smooth muscle cells were treated with ox-LDL in different concentration or different time. Based on that, the cells were pretreated with amlodipine at different concentrations （0, 0.1, 1.0 and 10.0 μmol/L） and then incubated with ox-LDL, RT-PCR and Western blotting were used to detected the expression of Cyclophilin A in the cells.Results Tile expression of Cyclophilin A in smooth muscle cells induced by ox-LDL was obviously up-regulated by amlodipine in a dose-dependent manner. Conclusion Amlodipine may reverse the role of ox-LDL on the Cyclophilin A expression in smooth muscle cells.