黄芪和红花对脑缺血再灌注后大鼠神经细胞凋亡和天冬氨酸特异性半胱氨酸蛋白酶8的影响

The Effect of Radix Astragali and Safflower Injection on Neuron Apoptosis and Caspase-8 After Local Cerebral Ischemia/Reperfusion in Rats

目的研究黄芪和红花对脑缺血再灌注大鼠神经细胞凋亡和天冬氨酸特异性半胱氨酸蛋白酶8的影响。方法采用局灶性脑缺血再灌注模型,分假手术组、模型组、黄芪加红花组、黄芪组和红花组。术后分为12h、24h和48h3个时间点,行神经功能评分;缺口末端标记法观察凋亡的情况;免疫组织化学观察缺血周围区神经细胞天冬氨酸特异性半胱氨酸蛋白酶8表达强度。结果黄芪和红花均能改善大鼠脑缺血再灌注后的神经功能评分。缺血再灌注24h的凋亡阳性细胞数与模型组比较,各治疗组差异均有显著性(P<0.05);在治疗组中,黄芪加红花组与红花组和黄芪组凋亡阳性细胞数比较差异均有显著性(P<0.05)。天冬氨酸特异性半胱氨酸蛋白酶8阳性表达水平:缺血再灌注12h,黄芪组、黄芪加红花组与模型组比较,差异均有显著性(P<0.05);红花组与黄芪加红花组比较,差异均有显著性(P<0.05)。缺血再灌注24h和缺血再灌注48h,各组之间差异有显著性(P<0.05)。结论黄芪加红花可减轻大鼠脑缺血再灌注后神经细胞凋亡,其机制可能与下调凋亡通路中的天冬氨酸特异性半胱氨酸蛋白酶8的表达有关。

Ahn To study the effect of radix astragali（RA）and saffior injection on neuron apoptosis and caspase-8 following local cerebral ischemia/reperfusion. Methods Male adult rats were randomly divided into three groups： the sham-operated group, the model group, the RA group, the safflor group and the RA ＋ safflor group. The model of middle cerebral artery occlusion was established by thread ligation method. Neurological system symptoms were evaluated after 12 h, 24 h, 48 h of ischemia/ reperfusion. Then rats were decapitated at 12 h, 24 h, and 48 h after reperfusion, and brains were taken for TUNEL and immunohistochemistry examinations. Positive reacted cells of apoptosis and Caspase-8 are counted under light microscope at different time points of ischemia/reperfusion. Results RA and safflor can significantly alleviate neurological system damage; Apoptosis examination showed that positive reacted cell amount in the RA group, the safflor group and the RA ＋ safflor group was significantly milder than that in the model group （ P〈0.05 ）. And then, as compared with the RA group and the saffler group, amount was declined in RA ＋ safflor group （ P 〈 0.05 ）. Immune examination of Caspase-8 showed that positive reacted cell amount in the RA group, the safllor group and the RA ＋ safflor group was significantly milder than that in the model group （ P 〈 0.05 ）. And then, as compared with the RA group and the safflor group, amount was declined in RA ＋ safflor group （ P 〈 0.05 ）. Conclusions The protective effects of the RA and saftlor against cerebral ischemia/reperfusion injury might be associated with downing regulation of the Caspase-8 expression.