湖南汉族人群脂联素基因多态性分布及其与动脉粥样硬化性脑梗死和相关危险因素的关系

Relationship Between Adiponectin Gene Polymorphisms and Atherosclerotic Cerebral Infarction As Well As Risk Factors in Chinese Han Population of Hunan Area

目的探讨脂联素基因45T/G和276G/T多态位点在湖南地区汉族人群中的分布及其与动脉粥样硬化性脑梗死和相关危险因素的关系。方法收集163例正常对照者和161例动脉粥样硬化性脑梗死患者的血标本,聚合酶链反应限制片长多态性分析法检测脂联素基因45T/G和276G/T多态性在动脉粥样硬化性脑梗死组和正常对照组的基因频率,同时检测研究对象的血脂和血压水平。结果中国湖南地区汉族人群脂联素基因45T/G和276G/T位点等位基因频率分别是0.783/0.217和0.697/0.303。脂联素基因45T/G多态位点等位基因和基因型分布频率两组相比差异无统计学意义(P>0.05);动脉粥样硬化性脑梗死组脂联素基因276G/T多态位点TT基因型频率和T等位基因频率明显高于对照组(P<0.05),且TT基因型亚组甘油三酯水平较GG基因型亚组显著升高,差异有统计学意义(P<0.05)。结论脂联素基因45T/G多态性可能与中国湖南汉族人群动脉粥样硬化性脑梗死的发生无关;276G/T多态性可能参与中国湖南汉族人群动脉粥样硬化性脑梗死的发生,且可能影响血脂水平,276TT型者可能具有较高水平的甘油三酯。

Aim To investigate the relationship between adiponectin gene polymorphisms and the occurrence of atherosclerotic cerebral infarction （ACI）, blood pressure as well as the lipid concentrations in Chinese Han population of Hunan area. Methods 163 healthy individuals and 161 ACI patients were recruited in this study. The genotypes of adiponectin were detected with polymerase chain reaction （PCR） and digested by specific restriction enzymes. Results Adiponectin polymorphisms existed in Chinese Han population of Hunan area, with the allele frequencies 0. 783/ 0. 217 for 45T/G in the whole study population, and 0. 697/0. 303 for 276G/T, respectively. There was no significant difference of genotype and allele frequency between ACI patients and controls for 45T/G pelymorphisms （ P 〉0.05 ）. However, the frequency of T allele and TT genotype in adiponectin 276G/T polymorphisms were significantly higher in ACI group compared with that in control group （ P 〈0.05 ）. Also, T allele carriers had a significantly higher level of triglyceride （TG） than subjects with GG genotype. Conclusions 45T/G polymorphism of adiponectin might has no significant correlation with ACI in Chinese Han population of Hunan area, whereas 276G/T polymorphism might be associated with the development of ACI through a participation in the lipid metabolism.