嗜酸热异养菌TC-2的分离鉴定及其对微生物浸矿的影响

Isolation,characterization of heterotrophic acidothermophile TC-2 and its influence on bioleaching of chalcopyrite

从云南腾冲酸性热泉分离出一株嗜酸热异养菌TC-2。该菌呈长杆状,细胞大小为(2.0～5.0)μm×(0.3～0.5)μm,革兰氏染色反应为阴性,能形成芽孢。该菌生长温度范围为45～70℃,最适生长温度为55℃,适宜生长的pH值为1.5～4.0,最适宜生长的pH值为2.5,该菌能利用多种有机物生长。16SrDNA序列分析显示其与Alicyclobacillus sendaiensis的16SrDNA相似性大于99%。该菌自身没有浸矿作用,但对Acidianus manzaensis、Sulfolobus metallicus和Metallosphaera sedula等高温浸矿菌的浸矿有一定促进作用。

A heterotrophic acidothermophilic bacterium TC-2 was isolated from an acid spring sample collected in Tengchong, Yunnan, China. The gram-negative-staining cells of the strain were straight long rods, 2.0-5.0μm in length and 0.3-0.5 μm in diameter. The temperature range for growth was from 45 ℃ to 70 ℃ with an optimum temperature of 55 ℃. The pH range for growth at 55 ℃ was from 1.5 to 4.0 with an optimum at 2.5. This bacterium can use a wide variety of organic compounds to support fast growth, but can not utilize chalcopyrite as energy source. 16s rRNA gene Analysis reveal that this strain closely relates to Alicyclobacillus sendaiensis, with over 99% sequence similarity between each other. Co-bioleaching of chalcopyrite with Acidianus manzaensis, Sulfolobus metallicus and Metallosphaera sedula shows that the strain TC-2 can improve leaching rates of the sulphide minerals.