马拉色菌相关人群及正常人群耳耵聍中马拉色菌带菌情况的调查

Isolation of Malassezia spp.in cerumen of healthy human and Malassezia associated patients

目的调查马拉色菌相关人群及正常人群耳耵聍中马拉色菌带菌情况。方法用结晶紫染色法对96例被调查人群耳耵聍进行马拉色菌检测,同时作培养,并以标准株作对照,用生理生化方法将耵聍中分离到的79株马拉色菌进行分类。结果马拉色菌相关人群耳耵聍中马拉色菌的直接检出率为91.84%(45/49),培养阳性率为81.63%(40/49),其中厚皮马拉色菌8株(16.33%),合轴马拉色菌10株(20.41%),糠秕马拉色菌22株(44.90%)。正常人群耳耵聍马拉色菌直接检出率为89.36%(42/47),培养阳性率为78.72%(37/47),其中厚皮马拉色菌5株(10.64%),合轴马拉色菌8株(17.02%),糠秕马拉色菌23株(48.93%),斯洛菲马拉色菌1株(2.13%)。结论马拉色菌为正常人群及马拉色菌相关人群外耳道正常菌群,两组人群中马拉色菌的分离率和菌种分布无显著性差异。

Objective To investigate the constitution ofMalassezia spp. in cerumen of healthy human and Malassezia associated patients. Methods Fourty-seven healthy volunteers and 49 Malassezia associated patients were included. Cerumen was detected by direct microscopic examination using Gram stain, and samples were sent for isolation simultaneously. The standard 7 strains of Malassezia spp. were used as control. The isolates were identified to species by physiological and morphological characeristics. Results In cerumen of Malassezia associated patients, 45 （91.84%） were positive using direct microscopic examination, 40 （81.63%） strains were isolated, including 8 strains of Malassezia pachydermatis , 10 strains of Malassezia sympodialis , 22 strains ofMalasseziafurfur. In cerumen of healthy volunteers, 42 （89.36%） were positive using direct microscope examination, 37 （78.72%） strains were isolated, including 5 strains of Malassezia pachydermatis , 8 strains of Malassezia sympodialis , 23 strains of Malassezia furfur and 1 strain of Malassezia sloffiae . Conclusions Malassezia spp. were normal commensal in ceru- men of human and Malassezia associated patients. No difference of isolation rate and constitution of Malassezia spp. in cerumen were detected in these two groups.