DNA芯片检测乙型肝炎病毒基因型

Genotyping of hepatitis B virus by DNA microarray

目的制备检测HBV基因型的DNA芯片并进行杂交验证。方法设计多条HBV分型寡核苷酸探针，固定于醛基基片的特定位置，制备成芯片，采用酶呈色技术（BCIP／NBT显色）检测杂交信号，进行杂交验证分析。结果106例临床样本均测出基因型，其中B型37例，C型69例；对所有样本进行测序分析，结果与芯片杂交一致，杂交的灵敏度和重复性等指标均佳。结论DNA芯片检测HBV基因型，操作简便易行，技术要求不高，适于临床推广应用。

Objective To prepare the microarrays for detection of HBV genotypes. Method HBV PCR products were hybridized with ohgonuleotide probes, which were prestablized on the chip. The hybridized results were colorized by BCIP/NBT. According to the hybridization signal and the corresponding probe sequence, HBV genotypes were determined. Results In the analysis of 106 HBV patients＇ serum samples using our developed microarray, we identified 37 genotype B, 69 genotype C. Sequencing results of all samples agreed 100% with microarray data. The hybridized signals on gene chips indicated that the sensitivity and specificity of DNA microarray for detecting the HBV genotypes were sarisfactory. Conclusions This assay was a quick, simple and effective method for HBV genotyping in chnic.