巨噬细胞中ABCA1表达及曲格列酮干预的影响

Expression of ATP-binding cassette A1 in macrophages treated with troglitazone

目的研究在不同的刺激因素及曲格列酮的干预作用下ATP结合盒转运体A1(ATP-binding cassette A1,ABCA1)在巨噬细胞中表达的变化,从而探讨ABCA1在糖尿病动脉粥样硬化中的作用机制及曲格列酮的防治作用。方法在体外模拟糖尿病状态,分别以高葡萄糖、高胰岛素或糖基化终末产物刺激巨噬细胞,以曲格列酮预处理,再以上述3种因素刺激,检测巨噬细胞中ABCA1 mRNA表达的变化。结果高葡萄糖、高胰岛素或糖基化终末产物均可抑制ABCA1 mRNA在巨噬细胞中的表达;曲格列酮预处理后,ABCA1 mRNA在巨噬细胞中的表达增加。结论糖尿病状态下的多种因素可抑制ABCA1 mRNA在巨噬细胞中的表达;曲格列酮可减弱这种作用,提示高糖等因素减少ABCA1 mRNA表达的作用可能与PPARγ有关。曲格列酮可能延缓糖尿病患者患动脉粥样硬化。

Objective To investigate the effects of various stimulating factors and troglitazone on the expression of ATP-binding cassette A1 （ ABCA1 ） in.macrophages, and explore the mechanism of ABCA1 on diabetic atherosclerosis, and the effect of troglitazone on diabetic atherosclerosis. Methods Diabetic state in vitro was mimicked, high glucose （HG） ,high insulin （HI） ,and advanced glycosylation end products （AGE） with or without troglitazone pre-treatment were used to detect the mRNA expression of ABCA1 in macrophages. Results The expression levels of ABCA1 mRNA in macrophages were significantly lower in HG group, HI group or AGE group compared with control group, and were significantly increased after troglitazone pre-treatment. Conclusion The mRNA expression of ABCA1 can be inhibited by these stim- c ulating factors on diabetic state, and can be markedly blocked by troglitazone;Troglitazone can delay the process of diabetic atheroselerosis.