实时荧光定量PCR技术及其在昆虫学研究中的应用

Real-time fluorescent quantitative PCR technique and its applications in entomological studies

实时荧光定量PCR(real-time fluorescent quantitative polymerase chain reaction,FQ-PCR)是一种利用荧光信号实时监测体外DNA分子PCR复制过程中每个循环的扩增产物,从而实现对DNA模板进行定量分析的技术,具有准确、快速、灵敏、特异等优点,在动植物基因工程、动植物检疫、微生物鉴定与分类、食品安全检测和医学等领域中得到广泛应用。本文对实时荧光定量PCR技术的原理、优缺点及近年来新的荧光探针的原理、类型进行了评述,并对实时荧光定量PCR技术在昆虫学研究中的应用进行了综述。

Real-time fluorescent DNA template concentration quantitative PCR is a laboratory technique monitoring the fluorescent signal in each Sciences, Zhejiang Medical University, for quantitative measurement of the of DNA amplification cycle. This technique is accurate, speedy, sensitive and specific. It has been widely used in such fields as bioengineering, quarantine, microbe identification and classification, food safety inspection and medical science. In this article, the principles, characteristics and recent advances in fluorescent probes of the realtime fluorescent quantitative PCR assay, and its applications in entomological studies were reviewed.