乳酸杆菌对自身免疫病模型小鼠免疫功能的影响

Effect of Lactobacillus rhamnosus GG on immune response： experiment with autoimmune mice induced by campylobacter jejuni

目的探讨乳酸杆菌（LGG）对空肠弯曲菌（CJ）致敏的自身免疫病模型小鼠（CJ小鼠）免疫功能的影响。方法ICR小鼠随机分为正常对照组、模型组、LGG组及氢化可的松（HC）组，除正常组外，其余组制备自身免疫病小鼠模型。从造模第15天起分别腹腔（或皮下）注射，连续用药7d，造模第25天处死小鼠。用酶联免疫吸附试验（ELISA）法测定小鼠血清中ds－DNA抗体水平及脾淋巴细胞的γ干扰素（IFN－γ）分泌活性；检测脾T、B淋巴细胞的增殖活性；镜下观察肝、肾组织的病理变化。结果LGG对ds—DNA抗体没有影响，LGG可改善脏器组织的病理性改变。LGG组CJ小鼠的T、B淋巴细胞增殖活性及脾淋巴细胞分泌IFN－γ活性明显低于模型组[0．42±0．05 vs 0．60±0．09，0．43±0．05 vs 0．60±0．09，（184±16）vs（195±6）pg／ml，均P〈0．01]。结论LGG对CJ小鼠的免疫机能有一定的免疫抑制作用。

Objective To investigate the immune effects of Lactobacillus rhamnosus GG （LGG） on immune response. Methods Fourty ICR mice were randomly divided into 4 groups, normal control （ N ） group, model （ M ） group, Lactobacillus （ LGG ） group, and hydrocortisone （ HC ） group. The mice were induced by Campylobacter jejuni with complete Freund＇s adjuvant as autoimmune animal model except N group. From the fifteenth day after primary immunization, each group was given different administration through peritoneum route for 7 days. Twenty-five days after the model establishment, the mice were killed. Then the blood sample was collected the undergo ELISA to detect the level of ds-DNA antibody and interferon （IFN） -γ. The proliferation activities of the B and T cells were examined. The livers and kidneys were taken out to undergo microscopy. Results The ds-DNA antibody levels of the HC and LGG groups were not significant different from that of the M group （both P 〉 0, 05 ）. The proliferation activities of T and B cells and IFN-γ level of the groups LGG were all significantly lower than those of the group M [ 0.42 ± 0.05 vs 0. 60 ±0.09,0. 43 ± 0. 05 vs 0. 60± 0. 09, （ 184 ± 16） vs （ 195 ± 6） pg/ml, all P 〈 0. 01 ]. Conclusion I,GG inhibits the immune response induced by CJ to a certain degree.