摘要
在二不同精力底层,元素的硫和铁的硫酸盐上种,有选择地与热水处理被准备并且清楚地由二维的胶化电气泳动出现的 Acidithiobacillus ferrooxidans 的细胞外的蛋白质的部分。某蛋白质比在铁的硫酸盐精力在硫精力底层与显然更高的丰富看到底层被使用 MALDI-TOF/TOF 识别。基于肽团指纹和 bioinformatical 分析,细胞外的蛋白质作为夫妇的转移蛋白质, pilin, vacJ 脂蛋白,多糖 deacetylase 家庭蛋白质, Ser/Thr 蛋白质磷酸酶家庭蛋白质和假想蛋白质根据他们的功能被分类。几细胞外的蛋白质在 thiol 组并且与 CXXC 功能的主题被发现丰富,这些蛋白质可以被他们的 thiol 组的使用直接涉及硫激活(Pr 嘘) 结合元素的硫。
The fractions of the extracellular proteins of Acidithiobacillus ferrooxidans grown on two different energy substrates, elemental sulfur and ferrous sulfate, were selectively prepared with hot water treatment and distinctly shown by two-dimensional gel electrophoresis. Some protein spots with apparently higher abundance in sulfur energy substrate than in ferrous sulfate energy substrate were identified by using MALDI-TOF/TOE Based on peptide mass fingerprints and bioinformatical analysis, the extracellular proteins were classified according to their functions as conjugal transfer protein, pilin, vacJ lipoprotein, polysaccharide deacetylase family protein, Ser/Thr protein phosphatase family protein and hypothetical proteins. Several extracellular proteins were found abundant in thiol groups and with CXXC functional motif, these proteins may be directly involved in the sulfur activation by use of their thiol group (Pr-SH) to bond the elemental sulfur.
出处
《中国有色金属学会会刊:英文版》
EI
CSCD
2008年第6期1398-1402,共5页
Transactions of Nonferrous Metals Society of China
基金
Project(50674101)supported by the National Natural Science Foundation of China
Project(2004CB619201)supported by the National Basic Research Program of China
Project(50621063)supported by the Chinese Science Foundation for Distinguished Group
关键词
氧化硫磺
细胞外蛋白质
氧化亚铁
生物技术
sulfur oxidation
extracellular proteins
Acidithiobacillus ferrooxidans