细胞因子及其受体基因多态性与Ⅲ型前列腺炎关系的初步探讨

Association of Gene Polymorphisms of Cytokine and Cytokine Receptor with Type Ⅲ Prostatitis

目的：探讨细胞因子及其受体基因多态性与Ⅲ型前列腺炎发病的关系。方法：我院泌尿外科门诊Ⅲ型前列腺炎患者24例，年龄24～40岁，平均31岁，51例健康志愿者为正常对照组，年龄22—40岁，平均28岁。采用序列特异性引物聚合酶链反应（SSP—PCR）方法检测受试者外周血13种细胞因子和细胞因子受体22个位点的基因型。结果：患者和对照组比较，IL-10（-819C／T）位点T／T基因型（62．5％绑31．3％）多见，IL-10（-592C／A）位点A／A基因型（62．5％vs31．3％）多见；IL-10（-1082，-819，-592）位点ATA单倍体型多见（75．0％vs55．9％），ATA／ATA二倍体基因型多见（62．5％vs31．2％），差异均有统计学意义（P〈0．05）。结论：抗炎细胞因子IL-10基因多态性与Ⅲ型前列腺炎发病有关。

Objective ： To explore the role of the gene polymorphism of cytokine and cytokine receptors in the pathogenesis of type Ⅲ prostatitis. Methods： We genotyped 24 outpatients diagnosed with type Ⅲ prostatitis and 51 healthy volunteer controls for the single nucleotide polymorphisms of 13 cytokines and cytokine receptors at 22 sites by Sequence Specific Primer -PCR （SSP-PCR）. Results ： The patients exhibited significantly higher frequencies of the genotypes of IL-10-819 T/T （62.5%） and IL-10-592 A/A （62.5%）, the haplotype of IL-10 （-1082/-819/-592） ATA （75. 0% ） and the diploid genotype of IL-10 （-1082/-819/-592） ATA/ATA （62.5%）, than with the healthy controls （31.3%, 31.3%, 25.0% and31.2%） （P〈 0.05）. Conclusion： Our data suggested that anti-inflammation cytokine IL-10 gene polymorphisms were associated with the pathogenesis of type Ⅲ prostatitis.