吸烟对大鼠阴茎血管内皮细胞P-选择素表达的影响

Expression of P-selectin in the Penile Vascular Epithelia of Smoking Rats

目的:观察不同吸烟量大鼠阴茎血管内皮细胞P-选择素的表达和海绵体超微结构的改变,初步探讨吸烟导致阴茎勃起功能障碍的发病机制。方法:健康Wistar大鼠50只,随机均分为正常对照组、长期大量吸烟组、长期小量吸烟组、短期大量吸烟组和戒烟组。皮下注射阿朴吗啡(APO)检测阴茎勃起功能。采用ELISA法测定大鼠阴茎血管内皮细胞P-选择素的水平,透射电镜观察大鼠阴茎海绵体超微结构的变化。结果:正常对照组P-选择素为(10.78±1.71)ng/L;长期大量吸烟组为(62.62±5.95)ng/L;长期小量吸烟组为(40.06±3.97)ng/L;短期大量吸烟组为(41.37±4.06)ng/L;戒烟组为(22.80±3.15)ng/L。4组与对照组比较均有显著性差异(P<0.05)。电镜观察吸烟各组内皮细胞、血窦、平滑肌细胞分布杂乱,血管内皮细胞连续性破坏;内皮细胞和平滑肌细胞超微结构被破坏。大量间质组织增生、纤维化。结论:吸烟可致阴茎血管内皮细胞P-选择素表达增加,破坏海绵体组织超微结构,可能是吸烟致阴茎勃起功能障碍的主要途径之一。

Objective： To observe the expression of P-selectin in the penile vascular epithelial cells and the morphological changes in the uhrastrueture of the penile cavernous tissues of smoking rats, and to explore the pathogenesis of smoking-induced erectile dysfunction. Methods： Fifty healthy Wistar rats were randomly divided into a normal control, a long-term heavy smoking group, a long-term light smoking, a short-term heavy smoking and a smoking cessation group. Their erectile function was tested by subcutaneous injection of apomorphine （APO）, the P-seleetin expression in the penile vascular epithelial cells detected by ELISA and the morphological changes in the ultrastrneture of the penile cavernous tissues observed under the transmission electron microscope （TEM）. Results ： The levels of P-selectin were 10.78 ±1.71 ng/L, 62.62 ± 5.95 ng/L, 40.06 ±3.97 ng/L, 41.37 ± 4.06 ng/L and 22.80 ± 3.15 ng/L respectively in the normal control, long-term heavy smoking, long-term light smoking, short-term heavy smoking and smoking cessation groups, with significant differences between the control group and the other four （ P 〈 0.05 ）. Electron microscopy showed abnormal arrangement of endothelia, penile cavernous sinuses and smooth muscle cells, disrupted continuity of endothelia, damaged ultrastrneture of endothelial and smooth muscle cells in the penile cavernous tissue, and obvious proliferation and fibrosis of interstitial tissues in the smoking rats. Conclusion ： Smoking increases the P-selectin expression in the penile vascular epithelial cells and damages the ultrastructure of the penile cavernous tissue, which may be the main contributors to smoking-induced erectile dysfunction.