摘要
目的:探讨Y染色体上AZF微缺失和反复自然流产之间的关系。方法:收集26份男性流产胚胎的绒毛样本,51份配偶反复流产的男性血液,分别提取基因组DNA,选取位于Yq11.2上AZFa、AZFb和AZFc的12个STS位点,对流产胚胎绒毛样本和血液样本进行AZF的多重PCR检测。结果:流产胚胎的26份样本中没有发现AZF的微缺失,51份血液样本中也没有发现AZF的微缺失。结论:排除AZF微缺失为反复自然流产的原因。
Objective： To study the relationship between azoospermia factor （AZF） microdeletions of the Y-chromosome and recurrent spontaneous abortion. Methods： We collected 26 chorionic villus samples from abortive male embryos and 51 blood samples from the husbands whose wives had recurrent spontaneous abortion, extracted genomic DNA from the samples and detected 12 STSs in the AZFa, AZFb and AZFc regions of Yq11.2 by amplification multiplex PCR. Result： AZF microdeletions were found neither in the chorionic villus samples nor in the blood samples. Conclusion ： There is no relationship between AZF microdeletions and recurrent spontaneous abortion.
出处
《中华男科学杂志》
CAS
CSCD
2008年第12期1099-1102,共4页
National Journal of Andrology
关键词
无精子症因子
反复自然流产
多重PCR
azoospermia factor
recurrent spontaneous abortion
multiplex PCR
