超重人群皮下和网膜脂肪组织中脂联素基因表达的研究

Study on Adiponectin Gene Expression of Subcutaneous and Omental Adipose Tissues in Subjects with Obesity

目的:探讨脂联素(APN)在皮下(SC)和网膜(OM)脂肪组织中的mRNA表达水平与肥胖的关系。方法:实时荧光定量PCR法检测48例超重[体质量指数(BMI)≥25kg/m2)]和40例正常体质量人群(BMI<25kg/m2)腹部SC和OM脂肪组织中APN mRNA的表达,并测定血糖、胆固醇和HOMA-IR等生化指标。结果:在整体人群中,OM脂肪组织APN mRNA表达量均明显高于SC脂肪组织(P<0.05);OM脂肪组织APN mRNA表达量与BMI、腰臀比(WHR)和胆固醇(TC)水平呈负相关(r分别为-0.249、-0.143、-0.148,P<0.05);SC脂肪组织APN mRNA表达量与BMI、WHR、TC和三酰甘油(TG)水平呈负相关(r分别为-0.281、-0.180、-0.247、-0.172,P<0.05),并与同一个体的OM脂肪组织APN mRNA表达量呈正相关(r=0.192,P<0.05)。结论:APN的基因表达量内脏高于外周,提示循环APN可能主要来源于内脏脂肪组织。

Objective： To explore the relationships between obesity and adiponectin mRNA expression of subcutaneous （SAmR） and omental （OAmR） adipose tissues. Methods： SAmR and OAmR of 48 subjecets with obesity [body mass index （BMI） ≥25 kg/m2）land 40 subjecets with normal weight （BMI 〈 25 kg/m2）were measured by real-time quantitative RT-PCR with TaqMan probe. Plasma glucose, cholesterol （TC） and insulin resistance index by HOMA （HOMA-IR）were determined. Results： In the whole subjects, OAmR was significantly higher than SAmR （P 〈 0.05）. Significant, inverse correlation were observed between OAmR and BMI, waist to hip ratio （WHR） and TC （ r = -0.249, r = -0.143, r = -0.148, P〈 0.05）. SAmR correlated negatively with BMI, WHR, TC and TG （ r = -0.281, r = -0.180, r = -0.247, r = -0.172, P 〈 0.05 ）, and correlated positively with OAmR in the same subject （ r =0.192, P 〈 0.05）. Conclusion： Adiponectin mRNA expression of visceral adipose tissues is higher than that of periphery. The principal resource of circulating APN is probably from visceral adipose tissues.