摘要
目的研究Toll样受体2(Toll—like receptor 2,TLR2)和Toll样受体4(Toll—like receptor 4,TLR4)在真菌性鼻及鼻窦炎(fungal rhinosinusitis,FRS)患者鼻黏膜中的表达,并探讨其在FRS发病中的可能作用。方法采用逆转录-聚合酶链反应和免疫组化SP法分别检测24例FRS、24例慢性鼻及鼻窦炎(chronic rhinosinusitis,CRS)和15例鼻中隔偏曲手术患者正常下鼻甲鼻黏膜中TLR2和TLR4 mRNA和蛋白的表达水平。结果TLR2和TLR4 mRNA在FRS组的表达水平(0.208±0.063和0.1 59±0.059)明显低于CRS组(0.505±0.097和0.406±0.102)和鼻中隔偏曲患者组(0.327±0.047和0.232±0.088,P均<0.05)。FRS组TLR2和TLR4蛋白的阳性表达率明显低于CRS组和鼻中隔偏曲组(P<0.05)。结论TLR2和TLR4在FRS中表达下调,提示Toll样受体介导的天然免疫在鼻黏膜抗真菌防御反应中作用下降,是引起鼻黏膜真菌清除率下降和真菌易感性增加的原因之一。
OBJECTIVE To study the expression of Toll-like recptor2（TLR2） and Toll-like receptor4（TLR4） in the fungal rhinosinusitis tissues, and to explore the role of TLR2 and TLR4 in the pathogenesis of fungal rhinosinusitis. METHODS The mRNA and protein expression of TLR2 and TLR4 were detected by reverse transcription polymerase chain reaction （RTPCR） and immunohistochemistry （IHC） in 24 fungal rhinosinusitis tissues, 24 chronic rhinosinusitis tissues, and 15 normal mucosa of inferior turbinate. RESULTS The mRNA expression of TLR2 and TLR4 in fungal rhinosinusitis tissues （0.208±0.063, 0.159±0.059） was significantly decreased compared with that in chronic rhinosinusitis （0.505±0.097, 0.406±0.102） and normal inferior turbinate tissues （0.327±0.047; 0.232±0.088）. The protein expression rates of TLR2 and TLR4 in fungal rhinosinusitis tissues （3/24, 4/24） were significantly lower than that in chronic rhinosinusitis （20/24, 21/24） and normal inferior turbinate tissues （7/15, 8/15） by IHC study.CONCLUSION The lower expression of TLR2 and TLR4 in fungal rhinosinusitis tissues indicates that the declined role of TLR-mediated innate immune responses in the defensive reaction of anti-fungal in sinonasal mucosa, which may be one of the reasons for dropped clearance rate and increased susceptibility to fungi.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2008年第12期685-688,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
