下咽鳞癌Ets-1、磷酸化STAT3的表达及其与侵袭转移的关系

Expressions of Ets-1 and phosphorylated-STAT3 correlated with invasion and metastasis of hypopharyngeal carcinoma

目的检测转录因子Ets-1和磷酸化信号转导和转录活化因子3（P-STAT3）在下咽癌原发灶及其颈淋巴结转移灶的表达，并探讨其与下咽癌侵袭转移的关系。方法应用免疫组织化学SP法检测52例单纯手术患者下咽癌原发灶及相应36例颈淋巴结转移灶中Ets-1、P-STAT3蛋白的表达。结果在52例下咽癌原发灶中，Ets-1、P-STAT3蛋白的表达率分别为61．54％（32／52）和75．00％（39／52）；Ets-1、p-STAT3蛋白在，13～T4级下咽癌原发灶的表达率均显著高于T1—T2级（P〈0．05，P〈0．01）；在有颈淋巴结转移下咽癌原发灶的表达率均显著高于无淋巴结转移者（均P〈0．05）；Ets-1蛋白在有肺转移下咽癌原发灶的表达率显著高于无肺转移者（P〈0．05）；在36例有颈淋巴结转移下咽癌中，Ets-1、P-STAT3蛋白在转移灶的表达率分别为52．78％（19／36）和58．33％（21／36）；原发灶P-STAT3蛋白表达率显著高于转移灶（P〈0．01）。结论Ets-1和P-STAT3蛋白在下咽癌中均高表达，并与肿瘤T分级、颈淋巴结转移密切相关，Ets-1表达还与肺转移有关。Ets-1和P-STAT3有可能成为临床判断下咽癌侵袭性、转移潜能的有效指标。

Objective To explore expressions of transcription factor Ets-1 and phosphorylated-signal transducers and activators of transcription 3（P-STAT3） in hypopharyngeal primary carcinoma and metastatic cervical lymph nodes and to study their correlation with invasion and metastasis of hypopharyngeal carcinoma. Methods Immunohistochemical staining was adopted to determine expressions of Ets-1 and P-STAT3 protein in 52 cases who had radical resection of hypopharyngeal carcinoma and matched 36 metastatic lymph nodes. Results In 52 cases of hypopharyngeal carcinoma, expression rotes of Ets-1 and P-STAT3 protein were 61.54%（32/52） and 75.00%（39/52）. The positive rates of Ets-1 and P-STAT3 in T3 and T4 classification were significantly higher than those in T1 and T2 classification（ P 〈 0.05, P 〈 0.01）. The positive rates of Ets-1 and P-STAT3 in metastatic lymph nodes were significantly higher than those in non-metastatic lymph nodes（ P 〈 0.05）. The positive rate of Ets-1 in metastatic lung tissues was significantly higher than that in non-metastatic lung tissues （ P 〈 0. 05）. In 36 eases of hypopharyngeal carcinoma with metastatic lymph nodes, expression rates of Ets-1 and P-STAT3 protein in metastatic lymph nodes were 47.22% （17/36） and 58.33% （21/36）. The positive rate of P-STAT3 in primary carcinoma was significantly higher than that in metastatic lymph nodes （P〈0.01）. Conclusion Ets-1 and P-STAT3 are over-expressed in hypopharyngesl carcinoma and are elesely related to the T classification and cervical lymph node metastasis, expression of Ets-1 is also significantly related to lung metastasis. Ets-1 and P-STAT3 may be used as clinical markers to predict invasion and metastasis potency of hypopharyngeal carcinoma.