正常分娩中产妇血清神经肽、神经递质检测及结果分析

Detection and analysis of serum neuropetide and neurotransmitter levels in pregnant women during parturition

目的探讨产妇在正常分娩过程中神经肽、神经递质水平的变化,分析结果了解其与正常分娩疼痛的关系。方法收集50例正常分娩产妇分别在分娩前、分娩时及分娩后三组实验组的标本,采用酶联免疫法检测血清中P物质、β-内啡肽和多巴胺的含量。用统计学方法比较各检测指标在各实验组的关系。结果50例正常分娩产妇血清中的P物质分娩前、分娩时及分娩后的平均含量分别是186pg/ml、160pg/ml和166pg/ml,三组P物质水平相当(P>0.05),无统计学意义。β-内啡肽的平均含量分别是3.20ng/ml、3.52ng/ml和2.85ng/ml,三组β-内啡肽水平不同(P<0.05),有统计学意义。多巴胺的平均含量分别是183pg/ml、216pg/ml和116pg/ml,三组多巴胺水平明显不同(P<0.01),有极显著性差异;分娩后分别与分娩时、分娩前比较,产妇多巴胺的含量后者均明显升高(P<0.01)结论正常产妇血清神经肽P物质在分娩过程中无显著变化;神经肽β-内啡肽和神经递质多巴胺存在异常,动态观测产妇血清神经肽、神经递质的水平变化可能有助于正常分娩疼痛的指导。

Objective To study the changes of serum neuropetide and neurotransmitter levels and the relationship between these markers and the perception of pain during normal parturition. Methods The specimens from three groups（before delivery, at delivery and after delivery） were collected in 50 cases of pregnant women. Substance P （SP）, β-Endorphin and dopamine were detected by ELISA, then all markers were analysed by means of statistics.Resuits The mean concentrations of substance P were 186pg/ml, 160pg/ml and 166pg/ml respectively, no significant difference among the three groups（P〉0.05）, the mean concentrations of β-Endorphin were 3.20 ng/ml, 3.52 ng/ml and 2.85 ng/ml respectively, and with indicating significant difference in the three groups（P〈0.05）. The mean concentrations of dopamine were 183 pg/ml, 216 pg/ml and 116 pg/ml respectively, and with significant difference among the three groups （P〈0.01）; Depamine level of the after delivery group showed lower than that of the before delivery group and the delivery group （P〈0.01）. Conclusions SP is little effect on the expression during normal parturition, but β-Endorphin and dopamine might be involved in this process. Dynamic observation of these markers levels is possibly helpful to guidance the pain of pregnant women during normal parturition.