金莲花有效成分的分离纯化和谱学表征

Separation and Purification and Spectra Characterization of Active Components from Trollius chinensis Bunge

本文采用溶剂萃取、柱层析和重结晶技术，对金莲花的有效成分进行了分离和纯化。利用显色反应，FTIR，UV—Vis，MS，^1H和^13C NMR等谱学表征手段，对分离纯化出的四种金莲花有效成分进行了分析和鉴定。谱学表征结果表明，四种有效成分分别为：3’，4’，5，7-四羟基黄酮-8-C-β-吡喃葡萄糖甙（1，荭草甙），4’，5，7-三羟基黄酮-8-C-β-吡喃葡萄糖甙（2，牡荆甙），3，4-二甲氧基苯甲酸（3，藜芦酸）和β-谷甾醇（4）。

The active components from Trollius chinensis Bunge have been separated and purified by using the technique such as solvent extraction, column chromatography and re-crystallization. Four active components from Trollius chinensis Bunge have been analyzed and identified by color reactions and FTIR, UV-Vis, ^1H, ^13 C NMR, MS analysis. The results of spectra characterization showed that the four compounds 1,2,3,4 are 3＇,4＇ ,5,7-tetrahydroxyl-flavoone-8-C-β-glucopyranosyl （ 1, orientin） ; 4＇,5,7-trihydroxyl-flavone-8-C-β-glucopyranosyl （2, vitexin） ; 3,4-dimethoxyl Benzoic acid （ 3, veratric acid） and β-sitosterol （4）, respectively.