高效液相色谱法测定热带经济作物中的叶黄素

Determination of Lutein in the Tropical Economy Crops by HPLC

本文建立了用丙酮提取，用WatersC18固相萃取小柱进行净化的高效液相色谱（HPLC）法，对热带经济作物中的叶黄素进行含量测定，测定结果表明：叶黄素在1～100mg／L时峰面积与进样质量呈现良好的线性关系，线性相关系数r=0．9997，相对标准偏差（RSD）为1．5％～4．2％（n=6），试样加标回收率为97．2％-101％，最小检测浓度为0．01mg／kg。测定结果显示：该方法快速、灵敏、可靠，具有简便，重现性好的特点。

The lutein extracted with acetone from the tropical economy crops was determined by high performance liquid chromatograph （HPLC） method, and purified by the solid phase extraction with waters C18- The determining results showed that there was a good linear calibration curve in the range from 1 to 100 mg/L （ r = 0. 9997 ）. The relative standard deviation of sample was 1.5%-4.2% （n = 6）, and the recovery of added lutein was 97.2%-101%. The detection limit was 0.01 mg/kg. The results suggested that this method is rapid, sensitive, simple, accurate and reproducible.