无患子总皂苷含量的测定

Determination of the Content of Total Sapindus-saponins

以香草醛-冰醋酸-高氯酸为显色体系，采用分光光度法测定无患子总皂苷含量。结果表明，无患子总皂苷在478nm处有最大吸收，在100～300pg范围内吸光度与无患子皂苷质量呈良好的线性关系，其回归方程为：A=0.00388m～0．064，r=0．99963。平均加标回收率为104．56％，RSD为3．69％。该方法简便、准确、稳定，是一种快速测定无患子总皂苷的有效方法。

The content of total Sapindus-saponins is determined by spectrophotometry using vanillin-acetic acid-perchlo- rie acid as the chromogenie reagent. The results show that the maximum absorption wavelength of Sapindus-saponins is at 478 nm, and a good linear relationship in the range of 100-300 μg of the total Sapindus-saponins is found, and its regression equation shows to be A = 0. 00388 m - 0. 064 ,r = 0.99963. The average recovery and RSD is 104.56% and 3.69% respectively. This method is simple, accurate and stable, and it could be used for rapid determination of total Sapindussaponins.