糜酶途径对人脐动脉平滑肌细胞增殖及细胞内ERK1/2表达的影响

Effects of Chymase Pathway on the Proliferation of Human Umbilical Artery Smooth Muscle Cells and Expression of ERK1/2 in the Cells

目的从细胞和分子水平探讨糜酶途径对平滑肌细胞（SMC）增殖的影响及其可能机制。方法以血管紧张素Ⅰ（AngⅠ）刺激SMC的生长，并通过卡托普利、糜酶抑素及氯沙坦分别阻断血管紧张素转换酶途径、麋酶途径及ATI受体，用MTT法检测各组SMC的增殖情况，用Westernblot检测各组SMC内细胞外信号调节激酶1／2（ERK1／2）的表达变化。结果AngⅠ组、卡托普利组、糜酶抑素组、氯沙坦组与对照组相比SMC均增殖活跃（P〈0．05），糜酶抑素组、氯沙坦组与AngⅠ组相比明显抑制SMC的增殖（P〈0．05）。各组SMC内ERK1／2表达量无明显变化（P〉0．05）。结论糜酶对SMC的增殖起重要作用，其分子机制有待进一步研究。

Objective To study on the effects of chymase pathway on proliferation of smooth muscle cells （SMC） and its mechanisms at cellular and molecular level. Methods SMCs were stimulated with Ang Ⅰ, and incubated with captopril,chymostatin and losatan in order to block ACE pathway, chymase pathway and ATI receptor respectively. SMC＇s proliferation was detected by MTT and the ERK1/2 expression was detected by Western blot. Results The cells＇ numbers were increased in drug interfered groups compared with those in control group （P 〈 0.05 ）, cell proliferation of chymostatin and losatan group were obviously inhibited compared with that in Ang I group （P 〈0.05）. The expression level of ERK1/2 did not change significantly. Conclusion Chymase played an important role on proliferation of SMC, but its mechanisms should be further studied.