薜荔果实乙醇提取液抗氧化活性的初步分析

Prilimilary Study on Antioxidant Activity of Ethanol Extracts from Ficus pumila L.Fruit

以不同体积分数(20%～95%)乙醇为溶剂,室温下经超声波辅助浸提,获得薜荔果实乙醇提取液.分别采用还原力法、DPPH法、TEAC法和FRAP法评估了不同提取液的抗氧化活性,并用Folin-Ciocal-teu法测定了提取液中总酚的质量分数.结果表明:不同体积分数乙醇溶液获得的薜荔果实提取液表现出不同的还原力,清除DPPH.、ABTS.+能力及总抗氧化能力,乙醇体积分数对提取液的抗氧化活性影响显著,不同乙醇提取液的抗氧化活性从高到低依次为:80%,95%,60%,40%,20%,其中体积分数为80%的乙醇提取液的还原力、清除自由基及总抗氧化能力最强,在700 nm处吸光值为1.04,清除DPPH.半清除率质量浓度为2.20 mg·mL-1,TEAC值为2.36,FRAP值为157.1,此结果与其提取液中总酚的质量分数(2.50%)最高相一致.

Fruit of Ficus purnila L. was extracted with various concentration （20%- 95%） ethanol solution using ultrasonic at room temperature. The antioxidant activities of different extracts were evaluated by reducing power assay, DPPH assay, TEAC assay and FRAP assay, the total phenolics contents of the extracts were measured by Folin-Ciocalteu method. The results demonstrated that the antioxidant activities of different concentration ethanol extracts showed varying degrees of efficacy in four assays （reducing power, scavenging capacity on DPPH · and ABTS · ^＋, the total antioxidant capacity）. The concentration of ethanol solution significantly affected the antioxidant activities of extracts. Antioxidant activities of different ethanol extracts were descending in the following order： 80%, 95%, 60%, 40%, 20% ethanol, in which, 80% ethanol extracts had the highest antioxidant activities, the absorbance value at 700 nm was 1.04 , half scavenging efficiency concentration for DPPH · was 2.20 mg · mL^-1, the value of TEAC and FRAP were 2.36, 157.1, respectively. The results were in accordance with the highest total phenolics content （2.50%） of 80% ethanol extract.