HPLC法测定羟基喜树碱人血清白蛋白纳米粒中的药物含量

Determination of 10-hydroxycamptothecin in human serum albumin nanoparticles by HPLC

目的建立羟基喜树碱（10-hydroxycamptothecin，HCPT）人血清白蛋白纳米粒（humanserumalbuminnanoparticle，HSA—NP）中药物含量的测定方法。方法采用KromasilC18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-水（体积比45：55），流速1．0mL·min^-1，紫外检测波长266nnq，柱温35℃。结果在本实验条件下制剂中辅料对HCPT含量的测定无干扰，HCPT质量浓度在2．0～10．0mg·L^-1内线性关系良好（r=0．9999，n=5），HCPT平均加样回收率为100．2％，RSD为1．0％（n=9）。结论本法准确可靠、简便易行，可用于羟基喜树碱人血清白蛋白纳米粒（HCPT—HSA—NP）中药物含量的测定。

Objective To establish an HPLC method for the determination of 10-hydroxycamptothecin （HCFT） in human serum albumin nanoparticles. Methods The separation was performed with a Kromasil C18 column （ 250 mm × 4.6mm, 5μm） at 35℃, and the mobile phase was methanol-water （ V： V = 45： 55 ）, the flow rate was 1.0 mL. min - 1. HCPT was detected at 266 nm. Results The linear range of HCPT with peak area was 2.0 - 10.0 mg- L -1 （ r = 0. 999 9, n = 5 ）. The average recovery of HCPT was 100.2%, and RSD was 1.0% （ n = 9）. Conclusions An accurate and simple method is established for the determination of HCPT in human serum albumin nanoparticles.