人类白细胞抗原DRB1位点基因多态性与内蒙古蒙汉民族人群哮喘关系的研究

Association between human leukocyte antigen DRB1 gene polymorphisms and asthma among Mongolian and Han peoples in China

目的探讨内蒙古蒙、汉民族人群中人类白细胞抗原HLA—DRB1基因多态性与支气管哮喘的关系。方法选用序列特异性引物聚合酶链反应（PCR—SSP）方法对内蒙古汉族支气管哮喘患者45例、蒙古族支气管哮喘患者45例进行HLA—DRB1等位基因的检测，并分别与46例健康汉族和45例健康蒙古族进行比较，计算各个位点的基因分布频率，相对危险度。对结果进行X^2检验，筛选有意义基因。结果蒙、汉族支气管哮喘组HLA—DR9基因频率分别为13．33％和16．67％，较相应对照组（2．22％和5．43％）增高（P〈0．05），比值比（OR）分别为7．82和4．10。蒙古族支气管哮喘患者HLA—DR11基因频率10．00％，较蒙古族正常对照组（1．11％）升高（P〈0．05），OR值为11．00。汉族支气管哮喘患者DR7基因频率较汉族正常对照组降低（P〈0．05），OR值为0.08。汉族支气管哮喘患者HLA—DR7基因频率1．11％，低于蒙古族支气管哮喘患者（10．87％），P〈0，05。结论HLA—DR9基因与蒙、汉族支气管哮喘的发病相关，是内蒙古地区蒙、汉族支气管哮喘患者共同的易感基因。而DR11基因位点是蒙古族支气管哮喘患者特有的易感基因，DR7基因位点是汉族支气管哮喘患者的抗性基因。

Objective To investigate the association between human leukocyte antigen DRBlgene （HLA-DRB1） polymorphisms and asthma among Mongolian and Han peoples in China. Methods Sequence-specific primer polymerase chain reaction （PCR-SSP） was used to detect frequencies of HLA DRB1 alleles in 45 Han and 45 Mongolian patients of asthma, as well as 46 healthy Han and 45 healthy Mongolian people as controls. Frequencies of each locus of HLA-DRB1 gene were calculated and odds ratio （OR） for each locus was estimated and tested by chi-square test. Results Frequency of HLA-DR9 allele was significantly higher in Han （13.33 % , OR of 7.82） and Mongolian （16. 67 %, OR of 4. 10） asthmatic patients, as compared to Han （2. 22 % ） and Mongolian （5.43 % ） controls, respectively （P 〈 0. 05 ） ; frequency of HLA-DR11 allele in Mongolian asthmatic patients （ 10. 00 % ） was significantly higher than that in healthy Mongolians （ 1.11% ） （ P 〈 0. 05, OR of 11.00 ） ; and frequency of HLA-DR7 allele in Han asthmatic patients was significantly lower than that in healthy Han people （ P 〈 0. 05, OR of 0.08 ）. Frequency of HLA-DR7 allele in Han asthmatic patients （ 1.11% ） was significantly lower than that in Mongolian patients （10. 87 % ）. Conclusions HLA-DR9 allele is a common in the people of Inner Mongolian Autonomous Region, and associated with susceptibility of asthma in Han and Mongolian peoples. HLA-DR11 allele is a unique susccptive gene for Mongolian asthmatic patients and HLA-DR7 allele is a protecting one for asthma in Han people.