高脂饮食对大鼠脂肪组织脂解调节因素的影响

Effect of Fat-Rich Diet on Lipolytic Regulatory Factors of Rats

目的:研究高脂饮食对大鼠脂肪组织HSL和perilipin蛋白表达及脂肪水解的影响。方法:将116只3周龄SD雄性大鼠随机分为普食组(C组)和高脂组,10周后形成高脂饮食诱导肥胖大鼠(OB组)和肥胖抵抗大鼠(OR组)。测试大鼠形态、血清、脂肪组织HSL和perilipin蛋白表达等指标。结果:OB组大鼠体重、脂肪重量、血清FFA和甘油等明显高于C组和/或OR组;OB组大鼠脂肪组织HSL活性和蛋白表达显著低于C组和OR组;而OB组大鼠perilipin蛋白表达显著高于C组,但显著低于OR组。结论:高脂饮食导致大鼠肥胖与其脂肪组织HSL活性和蛋白表达下调以及perilipin蛋白表达上调,因而与促进脂肪储存有关。

Objective： Research the effect of high - fat diet on hormone sensitive lipase and perilipin protein expression as well as the lipolysis of rat adipose tissue. Methods ： 116 SD male rats of three - week old are randomly divided into general diet control group （C Group） and high -fat diet group. After ten weeks, high -fat food is used to create obese rats （OB Group） and obesity- resistant rats （OR Group）. The morphology, serum and adipose tissue HSL and perilipin are tested. Results： The weight, fat weight, serum FFA and serum glycerol are significantly higher than those in rats of C group or OR group; the adipose tissue HSL activity and protein expression of OB group rats are markedly lower than those in the C group; while the adipose tissue perilipin protein expression of OB group are markedly higher than those in C group, but significantly lower than those in OR group. Conclusion ： The mechanism of obese rats induced by high - fat diet may be involved in the down - regulation of HSL activity and protein expression as well as the up - regulation of perilipin protein expression which promote fat storage.