绞股蓝多糖的组成分析及其对质粒DNA的保护作用

Polysaccharide from Gynostemma entaphyllum and its protection effect on plasmid DNA damage

目的:提取分离从陕西省平利县采集的绞股蓝中的多糖成分,分析其组成和对质粒DNA的保护作用。方法:绞股蓝经水提醇沉、透析和脱蛋白后得多糖,将多糖酸水解并用1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化后进行高效液相色谱分析;同时,观察绞股蓝多糖体外对过氧化氢(H2O2)光解反应诱导质粒DNA损伤的保护作用。结果:绞股蓝多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖8种单糖组成;绞股蓝多糖能保护DNA免受损伤,这种保护作用呈浓度依赖关系。结论:本试验方法简便灵敏,结果准确可靠,为综合开发利用绞股蓝提供了科学依据。

AIM： To analyse monosaccharides of gynostema polysaccharide from Gynostemma pentaphyllum gathered from Pingli County, Shanxi Province and study the protecting effect of polysaccharide on plasmid DNA damage. METHODS： Gynostema polysaccharide was extracted with hot distilled water. After dialysis and deproteinization, polysaccharide in trifluoroacetic acid solution was hydrolyzed by 1-phenyl-3-methyl-5-pyrazolone, and then determined by HPLC. Tolerance of gynostema polysaccharide to the UV-photolysis of H2O2 was used to investigate the protecting effect. RESULTS： Polysaccharide consisted of mannose （Man）, rhamnose （Rha）, glucuronic acid （GlcUA） , galacturonic acid （GalUA）, glucose （Glc）, galactose （Gal）, xylose （Xyl） and arabonose （Ara）. And it had the effect on protecting plasmid DNA from damage, showing a concentration dependent velationship. CONCLUSION： The method is simple and sensitive, the result is precise and believable. It provides scientific data for the use of C,7nostemma pentaphyllum.