摘要
采用玉米-豆粕型饲粮研究铜(源于硫酸铜)对肉鸡肝细胞线粒体呼吸链复合物活性的影响。在对照组(Cu 11 mg/kg,称试验组A)基础上设高铜组3个(含Cu分别为110,220和330 mg/kg,分别称为试验组B、C、D),试验期60 d。在12,24,36,48,60日龄采集肝脏,提取线粒体,测定线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ活性变化。结果表明,A组和B组线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ活性呈增加趋势;C组和D组活性有减弱表现,尤其是48 d之后,比各组的试验前期下降明显(P<0.05)。说明高铜日粮在一定程度上对线粒体复合物活性是有利的,但过高的铜添加日粮(≥220 mg/kg)可降低肉鸡肝细胞线粒体呼吸链复合物功能活性,可以推测线粒体是机体铜过量状态下侵害的靶部位之一,而且能作用于线粒体各个复合物,造成线粒体呼吸功能减弱,呼吸链中电子传递发生故障,从而影响线粒体功能的正常发挥。
The experiment was conducted to understand the influence of high-copper (from Copper sulfate) diet on mitochondrial complex from broiler hepatocyte. Based on the control group (Cu 11 mg/kg, group A), three experimental groups were set up. The contents of Cu were 110 mg/kg (group B), 220 mg/kg (group C) and 330 mg/kg (group D), respectively. All of the broilers were fed with corn-soybean meal for 60 days, and the activities of mitochondrial complex from broilers' hepatocyte were assayed at 12, 24, 36, 48 and 60 day-old. The result showed that the high-copper diet had an obvious influence on liver mitochondrial complex. The activities of each complex in group A and B had a tendency of increasing, especially in group B ( P 〈 0.05 ), but the activities were subject to decrease in group C and D ( P 〈 0.05 ). The above changes were more significant on the later stage of whole experiment ( after 48 day-old). The results indicated that high- copper diet could be helpful to liver mitochondrial complex of broiler to some extent, but too high-copper diet ( I〉220 mg/kg) might degrade the activity of mitochondrial complex. It was presumed that mitochondria was the invasive target under the condition of copper excess, and when mitochondrial complex was damaged, respiratory function of mitochondria was decreased and the electron transfer in respiratory chain might break down.
出处
《畜牧与兽医》
北大核心
2009年第1期18-21,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30671550)
关键词
肉鸡
高铜日粮
线粒体复合物
活性
broilers
high-copper diet
mitochondrial complex
activity