银杏酸GA1对酪氨酸酶和黑色素瘤细胞的作用

Inhibitory Effect of Ginkgolic Acids GA_1 on Mushroom Tyrosinase and B-16 Cell

对银杏外种皮提取的银杏酸通过制备型HPLC进行分离纯化,得到同系物单体GA1.研究GA1对酪氨酸酶的作用机理和对黑色素瘤细胞B16的作用.结果表明GA1可抑制酪氨酸酶单酚酶活力,对酪氨酸酶双酚酶具有较强的竞争性可逆抑制作用.低浓度GA1对黑色素细胞没有明显的杀伤作用,能明显降低细胞中黑色素的表达,是良好的酪氨酸酶抑制剂.

In this paper,GA1 was purified from ginko episperm,extraction＇s ginkgo acid by preparative HPLC. The effects of GA1 on the activity of mushroom tyrosinase and the B16 melanoma cells were studied. Results indicated that GA1 may inhibit both the monophenolase activity and diphenolase activity. The kinetic analyses showed that the strong inhibition of GA1 on the diphenolase was reversible and competitive. GA1 is harmless to the melanophore in low concentration. Tiny concentration of GA1 was able to inhibit the melanin expression in B16 cell obviously. So GA1 is one of promising tyrosinase inhibitors in the future.