钝顶螺旋藻不同形态藻丝体的蛋白质表达和生理差异的比较研究

Study on Protein Expression and Physiological Differences Among Four Morphologic Filaments of Spirulina platensis

无论是在生产还是在实验室培养条件下,螺旋藻多形性变异现象普遍存在.本研究从钝顶螺旋藻(Spirulina platensis)中分离纯化获得了4种不同形态的藻丝体,分别是直线形藻丝体(spL)、低度螺旋形藻丝体(sps-L)、中度螺旋形藻丝体(sps-M)和高度螺旋形藻丝体(sps-H).对这4种不同形态螺旋藻的生长速率、光合作用、叶绿素a(Chl a)含量分别进行研究,发现这4种不同形态藻丝体在生长和光合生理上存在明显的差异.而总蛋白的SDS-PAGE分析也表明,不同形态藻丝体在蛋白的表达上也有差异,其中34 ku的蛋白只在直线形藻丝体spL中大量表达.

Polymorphic variation of Spirulina has frequently been observed under laboratory and industrial condition. In our laboratory conditions, four morphological filaments of Spirulina platensis, which were linear filament（spL）,loose spiraled filament（sps- L）, regularly spiraled filament（sps-M） and tightly spiraled filament （sps-H）, were respectively separated and axenically cultivated. Further research showed that there are significant differences in growth rate, photosynthesis,and content of chlorophyll a among four forms of filaments. Furthermore, SDS-PAGE analysis also showed some obvious differences in total proteins extracted respectively from four forms of Spirulina platensis. The 34 ku protein, which was highly expressed in the linear filaments, was almost absent from the helical filaments .