摘要
采用免疫组化法分析６０例结肠癌、结肠息肉及结肠炎标本Ｒｂ、ＣＤＫ４和ｐ１６的蛋白表达。ｐ１６表达随细胞分化程度降低而减少，结肠炎组织ｐ１６表达随细胞分化程度降低而减少，结肠炎组织ｐ１６表达高于癌和息肉组。Ｒｂ在癌组织和息肉组织中表达明显减少。ＣＤＫ４在三种病变组织中表达无显著差异。推测结肠癌变与ｐ１６／ＣＤＫ４复合物降低，对Ｒｂ磷酸化抑制减少，使细胞分裂增殖增加有关。
he expressions of p16,CDK4 and Rb protein were detected by immunoperoxidase paint in routine formalin fixed paraffin embedded colorectal tissue section from colorectal carcinoma,polyp and colitis.The expression of p16 was declined with the decrease of cellular differentation levels,and p16 expression in colitis was higher than that of the colorectal carcinoman and polyp.The expression of Rb in carcinoma and polyp was obviously decrease.The expression of CDK4 in the three kinds of tissue was of no significant differences.The results suggest that the colon carcinoma is associated with the decrease of p16/CDK4 complex,the decreased inhibition of Rb phosphorylation and enhance of differentiation and proliferation of cells.
关键词
肿瘤抑制基因
CDK4
P16
肿瘤抑制蛋白
结肠肿瘤
Colonic neoplasms Tumor inhibiting gene Cyclin-dependent kinase Retinoblastoma Protein p16
