2,4-滴丁酯·扑草净·异丙草胺乳油的高效液相色谱分析

HPLC of 2,4-D butylate·prometryn·propischlor emulsifiable concentrate

采用高效液相色谱法,以Kromasil C-18(200×4.6mm,5μm)为固定相,以四氢呋喃+水(磷酸调pH=3.3)=500+500(V+V)为流动相,流速1.0mL.min-1,检测波长270nm,柱温40℃,在同一色谱条件下同时测定2,4-滴丁酯.扑草净.异丙草胺乳油中的2,4-滴丁酯、扑草净和异丙草胺。2,4-滴丁酯变异系数为0.62%,平均回收率为99.64%;扑草净变异系数为0.68%,平均回收率为99.81%;异丙草胺变异系数为0.48%,平均回收率为100.04%。

2,4 - D butylate, prometryn, propischlor in 2,4 - D butylate·prometryn · propischlor emulsifia- ble concentrate were determined under same chromatogram conditions which used Kromasil C -18（200 × 4.6mm, 5m） as stationary phase, butylene oxide ＋ water（ adjusted by phosphoric acid pH =3.3） =500 ＋500（ V ＋ V ） as mobile phase, flow rate was 1.0mL · min-1 , wavelengh was 270nm, column temperature was 40~C. The coefficient of variation and average recovery rate of three of them are as follows： 0.62%, 99.64% ; 0.68%, 99. 81% ; 0.48%, 100.04%.