大羽藓组培初代培养适宜消毒方法的筛选

Screening of Appropriate Disinfection Method of Early Cultured Thuidium cymbifolium Tissue Culture

[目的]为苔藓植物组织培养提供一些有价值的参考和依据。[方法]采用正交设计方法,对大羽藓配子体和孢子体组培初代培养消毒方法进行筛选。[结果]结果显示,配子体初代培养的最佳消毒方法为:0.5%次氯酸钠溶液+0.05%升汞消毒60 s;孢子体组培初代培养的最佳消毒方法为:用2.0%次氯酸钠消毒6 min。[结论]该试验筛选的方法比较适用于大羽藓配子体组培初代培养。

[Objective] The research aimed to provide valuable references for the tissue culture of bryophytes.[Method] Orthogonal design method was used to chose the appropriate disinfection method of early cultured Thuidium cymbifolium tissue culture.[Result] The results show that,the best way to disinfect the gametophyte in the early culture was： 0.5%sodium hypochlorite solution＋0.05% sercuric chloride,disinfecting for 60 s,and the best way to disinfect the sporophyte in the early culture was： use 2.0% sodium hypochlorite solution to disinfect for 6 min.[Conclusion] The methods screened in this study was suitable for the tissue culture of early cultured T.cymbifolium.