海南钻喙兰原球茎的形态建成和快速繁殖

Morphogenesis and Rapid Progagation of Protocorm of Rhynchostylis gigantea

[目的]为海南钻喙兰的组培快繁提供依据。[方法]以钻喙兰种子作外植体,研究了不同诱导培养基、增殖培养基、生根培养基在钻喙兰组培快繁中的效果。[结果]诱导类原球茎体以MS附加6-BA1.0 mg/L＋NAA0.1 mg/L的激素配比较好,分化时间短。增殖培养基为MS＋6-BA0～3.0 mg/L＋NAA0～0.2 mg/L,以MS＋6-BA3.0 mg/L＋NAA0.1 mg/L效果最好,平均增殖系数为3～8,把生长健壮并具有2～3片真叶的单个小芽,转接到生根培养基1/2 MS＋NAA1.0 mg/L上,经60 d培养,即可获得生长健壮的完整植株。钻喙兰试管苗的移栽基质以粗椰糠∶河沙=1∶1的比例混合较好,湿度80%～90%,移栽成活率达90%以上。[结论]海南钻喙兰种子在温度（25±2）℃,光照强度1 500～2 000lx,光照时间12 h/d的条件下,最适诱导培养基为MS＋6-BA1.0 mg/L＋NAA0.1 mg/L;最适增殖培养基为MS＋6-BA3.0 mg/L＋NAA0.1 mg/L,生根培养基为1/2 MS＋NAA1.0 mg/L。

[Objective] The aim was to provide the basis for the tissue culture and rapid propagation of Rhynchostylis gigantea in Hainan.[Method] With the seed of R.gigantea as explants,the effects of different induction medium,multiplication medium and rooting medium in the tissue culture of R.gigantea were studied.[Result] To induct the protocorm-like bodies,the MS medium added by hormone combination with 6-BA 1.0 mg/L and NAA 0.1 mg/L was better with short differentiation time.Multiplication medium was MS＋6-BA 0～3.0 mg/L＋NAA 0～0.2 mg/L and the effect of MS＋6-BA 3.0 mg/L＋NAA 0.1 mg/L was the best and its multiplication coefficient was 3～8.The healthy single particle with 2～3 true leaves was transplanted to rooting medium of 1/2 MS＋NAA 1.0 mg/L and after culture for 60 d the complete plant with healthy growth could be obtained.The transplanting medium of R.giganteatube seeding with crude coconut brain ∶ sand（1∶1） was better and the transplantation surviving rate could reached more than 90% under the moisture of 80%～90%.[Conclusion] Under the conditions of temperature of（25±2）℃,light intensity of 1 500～2 000 lx and illumination time of 12 h/d,the optimum induction medium of R.gigantea seed was MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L,its optimum multiplication medium was MS＋6-BA 3.0 mg/L＋NAA 0.1 mg/L and the rooting medium was 1/2 MS＋NAA 1.0 mg/L.