胆管上皮细胞与胆管癌细胞的核浆比值和嗜银核仁形成区相关蛋白的定量研究

A QUANTITATIVE STUDY ON NUCLEUS PLASMA RATIO AgNORs COUNTS OF CHOLANGIOCARCINOMA AND ATYPICAL EPITHELIAL HYPERPLASIA RELATED TO HEPATOLITHIASIS

采用图像分析技术定量分析胆管癌细胞与肝胆管结石所致胆管上皮不典型增生细胞核浆比值与核仁形成区相关蛋白的关系。结果显示：高、中、低分化胆管癌细胞与肝胆管结石胆管上皮单纯性增生细胞的核浆比值有显著性差异（Ｐ＜０．０１）；高分化与低分化胆管癌细胞核浆比值和嗜银核仁形成区相关蛋白计数有显著性差异（Ｐ＜０．０１）；但高分化胆管癌细胞与胆管上皮不典型增生细胞核浆比值无显著性差异（Ｐ＞０．０５）。由此提示，肝胆管结石所致胆管上皮增生可能是胆管癌的一种癌前病变。

Nucleus plasma ratio was measured and silver binding nucleolar organizer (AgNORs) were counted in 31 cases of cholangiocarinoma (11 cases were well differentiated, 10 case moderately differentiated and 10 cases poorly differentiated) and in 17 cases of atypical epithelial hyperplasia related to hepatolithiasis (9 cases were simple hyperplasia, 8 cases atypical epithelial hyperplasia) by AgNORs techique and image analysis.The results showed that mucleus plasma ratio and AgNORs counts increased significantly from well differentiated to poorly differentiated cholangiocarcinoma ( P <0.01). No statistically significant differance was shown between nucleus plasma ratio of atypical hyperplasia and well differentiated cholangiocarinoma.The data imply that chronic proliferative cholngitis in the presence of hepatolithiasis can progress to atypical epithelial hyperplasia which may be an important precursor of cholangiocarcinoma.